电化学发光免疫法与酶联免疫吸附法检测乙型肝炎病毒血清标志物的结果分析  

Analysis on the detection of HBV serologic markers by electrochemiluminescence immunoassay and enzyme-linked immunosorbent assay

作　　者：安静娜 李冬冬[1] 陈其霞[1] 王婷婷[1] 杨廷富[1] 陶传敏[1] 

机构地区：[1]四川大学华西医院实验医学科微生物实验室,四川成都610041

出　　处：《中国输血杂志》2015年第4期374-376,共3页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金(81072432/81301400);四川省科技厅科技支撑计划(2012FZ0126)

摘　　要：目的分析电化学发光免疫法(ECLIA)和酶联免疫吸附法(ELISA)检测乙型肝炎病毒(HBV)血清标志物的效果。方法回顾性分析同期16 451例ELISA法检测及15 541例ECLIA法检测的HBs Ag、抗-HBs、HBe Ag、抗-HBe、抗-HBc的结果,并收集205名临床患者的血清标本,采用2种方法检测,用Kappa检验判断各方法学检验结果的一致性程度。结果 ECLIA和ELISA法检测HBs Ag、抗-HBs、HBe Ag、抗-HBe和抗-HBc的符合率分别为100%,94.6%,92.2%,82.4%和74.6%。结论 ECLIA法与ELISA法检测HBV标志物符合率较好,但ECLIA法为半定量检测,敏感度更高,更适于在血站筛查以及临床手术等对结果极为敏感的情况下应用。Objective To analyze the results of HBV serologic markers detected by electrochemiluminescene immunoassay( ECLIA) and enzyme-linked immunosorbent assay( ELISA). Methods The results from the detection of 16 451 and 15541 samples by ECLIA and ELISA separately in the corresponding period were retrospectively analyzed. By using the two methods,205 cases of serum samples were detected. Kappa test was employed to determine the consistency of the results.Results The compliance rates between the ELISA and ECLIA were 100%,94. 6%,92. 2%,82. 4% and 74. 6% for HBs Ag,anti-HBs,HBe Ag,anti-HBe and anti-HBc,respectively. Conclusion Favorable compliance was observed between ECLIA and ELISA. ECLIA was a more favorable method in blood bank or under surgical situation due to its higher sensitivity.

关 键 词：电化学发光免疫分析法 酶联免疫测定法 乙型肝炎病毒 血清标志物 敏感度 

分 类 号：R446.61[医药卫生—诊断学]