分离白膜后血浆制备冷沉淀的质量评价  

Evaluation of the quality of cryoprecipitate prepared from the plasma separated from buffy coat

作　　者：李慧[1] 刘春燕[1] 李浩龙[1] 张锡敏[1] 王承琳[1] 杨振江[1] 

机构地区：[1]天津血液中心,天津300110

出　　处：《中国输血杂志》2015年第4期396-398,共3页Chinese Journal of Blood Transfusion

摘　　要：目的对采用22℃手工制备富含血小板白膜后分离的血浆制备冷沉淀的质量进行评价。方法 2013年11月—2014年6月,共随机选取22℃手工制备富含血小板白膜后分离的血浆70例,将其作为原料血浆制成冷沉淀后留样(即A组)。同时另随机选取4℃常规制备新鲜冰冻血浆90例,将其作为原料血浆制成冷沉淀后留样(即B组)。用全自动血凝分析仪检测冷沉淀中Ⅷ因子、纤维蛋白原含量,运用统计学方法对比分析2组有无统计学差异。结果 A组、B组Ⅷ因子含量分别为(136.96±59.32)IU、(163.53±77.33)IU(P<0.05),合格率分别为97%、99%(P>0.05)。A组、B组纤维蛋白原含量为(165.68±25.73)mg/袋、(164.11±17.54)mg/袋(P>0.05),合格率分别为91%和98%(P>0.05)。结论本文试验数据显示,22℃手工制备富含血小板白膜后分离的血浆可以用于制备冷沉淀。Objective To evaluate the quality of the cryoprecipitate obtained from the plasma which was separated from the platelet-rich buffy coat under the manual preparation at 22 ℃. Methods From November 2013 to June 2014,70 plasma samples separated from the platelet-rich buffy coat under manual preparation at 22 ℃ were selected randomly. The cryoprecipitate samples obtained from these 70 raw plasma samples were denoted as Group A. Meanwhile,additional 90 fresh frozen plasma samples under conventional preparation at 4 ℃ were selected randomly. These samples obtained from these 90 raw plasma samples were denoted as Group B. The fibrinogen and factor Ⅷ of the cryoprecipitate samples were detected by using automatic blood coagulation analyzer. The statistical difference between these two groups was also analyzed using a statistical approach. Results The factor Ⅷ contents of the Group A and Group B were(136. 96 ± 59. 32) IU and(163. 53 ± 77. 33)IU( P<0. 05),respectively. Their corresponding qualified rates were 97% and 99%( P<0. 05),respectively. Meanwhile,the fibrinogen contents of the Group A and Group B were(165. 68 ± 25. 73) mg / bag,(164. 11 ± 17. 54) mg / bag( P<0. 05),respectively. Their corresponding qualified rates were 91% and 98%( P>0. 05),respectively. Conclusion The plasma separated from the platelet-rich buffy coat under manual preparation at 22 ℃ could be used to produce qualified cryoprecipitate.

关 键 词：冷沉淀 新鲜冰冻血浆 富含血小板白膜 Ⅷ因子 纤维蛋白原 

分 类 号：R457.14[医药卫生—治疗学]