运用自配质控物比对分析干式生化仪ALT初筛结果的探讨  

Investigation on using self prepared quality control substance for comparison analysis of dry biochemical ALT screening

作　　者：熊志高 谭艳阳 易征 罗燕 

机构地区：[1]株洲市中心血站,湖南株洲412007

出　　处：《中国输血杂志》2015年第4期446-447,共2页Chinese Journal of Blood Transfusion

摘　　要：目的提高干化学法ALT检测质量,减少血液报废及血源流失。方法采用干化学法/速率法分别检测献血者全血和血清、室间质评质控物、自配质控物。结果干化学法检测全血、血清ALT结果基本一致;干化学法检测选用室间质评质控物,检测结果异常不可靠;自配质控物可用于干式生化仪的比对分析,每台仪器均产生不同的修正值。结论运用自配质控物对干式生化仪ALT检测结果进行修正,是可行且必要的;通过临界值修正等措施,ALT初筛准确性提高,ALT不合格报废率从1.18%降至0.53%。Objective To improve the quality of dry chemical method for ALT detection and to reduce blood waste and loss of source blood. Methods Whole blood and serum of blood donors were tested using dry chemistry method and enzymatic rate method,external quality control material and self-prepared quality control material. Results ALT detection results in whole blood versus serum using the dry chemical method were consistent and showed no variation. However,the results from the use of external quality control materials for dry chemistry detection of ALT were not reliable. In contrast,selfprepared quality control material can be used for comparative analysis performed by dry biochemistry analyzer. Each analyzer produced different correction values. Conclusion The self-preparatory quality control material is necessary and feasible for correcting the results from ALT detection performed by dry biochemistry analyzer. By correcting the threshold values,improvement could be seen in the accuracy of ALT screening while the blood discard rate could be reduced from 1. 18% to 0. 53%.

关 键 词：ALT 干化学法/速率法 比对分析 

分 类 号：R446.111[医药卫生—诊断学]