腐胺对白桦悬浮细胞中激素和黄酮积累影响的初步研究  

Effect of Putrescine on Hormone and Flavonoid Accumulation in Betula platyphylla Suspension Cultured Cells

作　　者：周文洋[1] 孙菲菲[1] 梁佳[1] 詹亚光[1] 范桂枝[1] 

机构地区：[1]东北林业大学生命科学学院,哈尔滨150040

出　　处：《中国农学通报》2015年第13期23-29,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目"PAs在真菌诱导白桦酯醇合成中的作用机理研究"(31100445);中央高校基本科研业务专项资金项目"信号分子对药用植物代谢物合成的调控作用研究"(DL13EA08-03);黑龙江省博士后科研启动金"PAs和H2O2在真菌促进白桦三萜合成中的cross-talk研究"(415288)

摘　　要：为明确腐胺对白桦悬浮细胞中激素和黄酮累积的影响,在白桦悬浮细胞生长的第8天添加不同浓度的腐胺、脱落酸(ABA)、茉莉酸甲酯(MeJA)、生长素(IAA)和玉米素核苷(ZR),采用紫外分光光度计法分析白桦悬浮细胞中黄酮含量和利用酶联免疫方法测定ABA、JA、IAA和ZR含量。外源腐胺和激素的添加均不同程度地提高了白桦悬浮细胞中黄酮的累积量,其中1mmol/L腐胺处理24h时增幅最高,增加了97.90%;激素中1mg/LABA处理后增幅最高,增加了103.40%。腐胺和激素共同处理后黄酮含量的增幅均较其单独处理提高。其中1mg/L腐胺与5mg/LJA处理后黄酮含量增幅最高,比对照和腐胺处理分别增加了197.43%和103.57%。同时腐胺处理后增加了IAA和ZR的含量,而降低了ABA和JA含量。进一步分析其相关性发现,腐胺处理后激素、激素比与黄酮含量的相关性增幅不同,其中JA与黄酮含量的相关性增幅最大,由对照的-0.883变为0.903。腐胺与ABA、JA、IAA和ZR协同促进了白桦悬浮细胞中黄酮的累积。The aim was to analyze the effect of exogenous putrescine on hormone and flavonoid accumulationin Betula platyphylla suspension cultured cells.Various concentrations of putrescine and exogenous hormonewere added to the eight-day-old suspension cultured cells,the content of flavonoid,ABA,JA,IAA and ZRwere analyzed by UV spectrophotometer and ELISA.Both exogenous putrescine and hormone treatmentincreased the content of flavonoid in birch suspension cultured cells.Among them,the highest flavonoidcontent was induced by 1 mmol/L putrescine for 24 h,the increasing rate was 97.90%.Amongthe hormone treatment,the highest flavonoid content was induced by 1mg ABA induction for 24 h,theincreasing rate was 103.40%.The joint treatment of putrescine and hormone increased much more flavonoidcontent than that of control or putrescine treatment.The highest flavonoid content was induced by 1 mmol/Lputrescine and 5mg/L JA,the increasing rate was 197.43% and 103.57% compared with control andputrescine treatment,respectively.At the same time,putrescine increased the content of IAA and ZR,butdecreased the content of ABA and JA.To further analyze the correlation,we found the correlation betweenhormones or hormone ratio with flavonoid content was different,JA treatment showed the highest change from ZR） regulated the flavonoid production in Betula platyphylla suspension cultured cells.-0.883 to 0.903.It can be inferred that the interaction between putrescine and hormone（ABA,JA,IAA andZR） regulated the flavonoid production in Betula platyphylla suspension cultured cells.

关 键 词：腐胺 白桦 黄酮 激素 

分 类 号：Q945.78[生物学—植物学]