叶用莴苣组织培养再生体系的建立  

Establishment of Tissue Culture Regeneration System of Leaf Lettuce

作　　者：黄冠华[1] 韩莹琰[1] 李雅博[1] 范双喜[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《中国农学通报》2015年第13期72-75,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析"(31372057);国家自然科学基金项目"叶用莴苣高温胁迫响应因子Ls Hsp70的功能研究"(31401883);现代农业产业技术体系建设专项"北京市叶类蔬菜创新团队"(blvt-02);北京农学院促进人才培养综合改革专项计划"叶用莴苣高温胁迫中LsHsp70家族基因的表达模式研究"(BNRC&GG201411);北京市科技新星计划项目"叶用莴苣耐热机理的研究及耐热品种的选育"(2010B020);蔬菜创新平台建设"蔬菜创新平台建设"(5075227039);北京市农业科技项目"生菜耐热新品种繁育及安全生产技术试验示范"(20140134)

摘　　要：为了满足市场需求,通过组织培养技术可以使叶用莴苣不受季节气候所牵制。随时可以工厂化大量生产,以达到百姓日食之需。本研究以PS11叶用莴苣为试材,从种子开始培育无菌苗,MS培养基为基础培养基,附加不同浓度的6-BA和NAA激素配比,再经愈伤组织诱导、芽分化、生根3个步骤的离体培养,建立叶用莴苣快速高效的再生体系。研究表明:该品种最佳诱导愈伤组织分化的培养基为MS+0.2mg/L6-BA+0.4mg/LNAA,出愈率达到100%,出芽率达到71.6%。In order to meet market demand, tissue culture technology is needed to lift the seasonal constrains of leaf lettuce production, and industrial mass production of the vegetable can satisfy people＇ s daily need. In this study PS11 of leaf lettuce was used as test material, aseptic seedling was bred from the seeds; MS culture medium was used as basic culture medium with supplemental different concentrations of 6-BA and NAA. Through the callus induction, bud differentiation and rooting, a fast and efficient regeneration system of leaf lettuce was established. The results showed that： the best medium for callus differentiation was MS＋0.2 mg/L 6-BA＋0.4 mg/L NAA, the callus induction rate reached 100%, and the budding rate reached 71.6%

关 键 词：叶用莴苣 组织培养 再生体系 

分 类 号：S636.2[农业科学—蔬菜学]