4个优良石榴品种叶片高频再生体系的建立  

Establishment of Adventitious Shoot Regeneration System from Leaves of 4 Pomegranate Cultivars in Vitro

作　　者：王菲[1] 陶吉寒[1] 尹燕雷[1] 冯立娟[1] 杨雪梅[1] 

机构地区：[1]山东省果树研究所,山东泰安271000

出　　处：《中国农学通报》2015年第13期100-107,共8页Chinese Agricultural Science Bulletin

基　　金：山东省国际科技合作项目"美国优质高效石榴良种引进及优异农艺关键基因的挖掘和功能定位研究"(2013GHZ31003);山东省果树研究所所长基金"石榴果实安石榴苷代谢生理机制研究"(2013KY04)

摘　　要：为提高石榴叶片再生率,增加再生芽数量及缩短再生周期,以‘泰山红’、‘泰山三白甜’、‘皮亚曼’、‘牡丹’4个石榴品种盆栽苗和无菌苗叶片为试材,研究培养基类型、生长调节剂配比、叶片来源和培养步骤对不定芽再生的影响。结果表明：MS是叶片再生最适培养基类型;无菌苗叶片比盆栽苗叶片更易再生不定芽;BA3.0mg/L＋KT1.0mg/L＋IBA0.3mg/L是叶片愈伤组织诱导、分化和不定芽形成过程中生长调节剂最佳配比;先在液体培养基上培养3-5天,再转至固体培养基比仅在固体培养基上效果更好,叶片再生率最高达98.47%,出芽数达7.51个。叶片不定芽的最佳单芽培养基和增殖培养基分别为B5＋BA0.6mg/L＋IBA0.5mg/L＋椰汁100mL/L＋PVP2.0g/L＋GA31.0mg/L和B5＋BA0.8mg/L＋IBA0.5mg/L＋椰汁100mL/L＋PVP2.0g/L,增殖系数为5.56。适宜的生根培养基为B5＋IBA1.0mg/L＋椰汁100mL/L,生根率为89.63%。由此建立了石榴叶片高频再生体系。Studies were carried out in order to improve regeneration rate of pomegranate leaves,increase thenumber of adventitious buds and reduce the regeneration cycle.The tested materials were the leaves of pottedseedlings and aseptic seedlings from 4 pomegranate cultivars‘Taishanhong＇,‘Taishansanbaitian＇,‘Pyaman＇and‘Peony＇.The effects of medium type,mixture ratio of growth regulator,source of leaves,and training stepson adventitious bud regeneration were investigated.The results showed that MS medium was the most suitablefor leaves regeneration,besides,leaves from aseptic seedlings produced shoot easier than that from pottedseedlings.BA 3.0mg/L＋ KT 1.0mg/L＋ IBA 0.3mg/L was the best mixture ratio of growth regulator in theprocess of leaves callus induction and differentiation and formation of adventitious buds.The leaves cultured inliquid medium for 3-5 days at first and then transferred to solid medium showed stronger regeneration abilitythan that cultured in the solid medium only.In this way,the ratio of leaf regeneration reached up to 98.47%,with an average of 7.51 adventitious shoots on each leaf.For leaves adventitious buds,the best single budculture medium and proliferation medium were B5＋ BA 0.6mg/L＋ IBA 0.5mg/L ＋ CW（coconut water）100mL/L＋PVP 2.0 g/L＋ GA3 1.0mg/L and B5＋ BA 0.8mg/L＋ IBA 0.5mg/L ＋ CW 100mL/L＋ PVP 2.0 g/L.The optimumrooting medium was B5＋ IBA 1.0mg/L＋ CW 100mL/L,of which the rooting rate was 89.63%.So the high frequency regeneration system from pomegranate leaves was established.

关 键 词：石榴 离体叶片 再生体系 不定芽再生 

分 类 号：S665.4[农业科学—果树学]