一种室内大量饲养灰飞虱的方法  

A Method for Mass Rearing of Small Brown Planthopper(Laodelphax striatellus ) in Laboratory

作　　者：任春梅[1] 程兆榜[1] 杨柳[1] 缪倩[1] 周益军[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国农学通报》2015年第13期182-186,共5页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金项目"灰飞虱地理种群的标记研究"(31201506);江苏省农业科技自主创新项目"灰飞虱地理种群的标记研究"[CX(13)5026]

摘　　要：灰飞虱是重要的农业害虫和病毒传播介体,为给相关研究提供足够的活体材料,建立了一种室内灰飞虱规模化饲养的方法,笔者经一系列生物学试验,对温度、光强、饲养密度等条件进行了优化,并对产卵和换苗时间进行了规定。试验表明饲养温度24-28℃,光照强度5000-7000 lx,不同龄期虫的饲养密度分别为300-400头/杯（若虫）、200-300头/杯（3-4龄虫）、100-200头/杯（成虫）适合各自饲养时水稻苗的生长、灰飞虱的发育繁殖、卵的孵化等。为保证同批次虫龄的一致性,规定产卵时间和换苗时间分别为48 h和8-10天。此方法条件因素明确,可操作性强,不受场地、时间、环境等自然条件限制;可随时提供足量试验用龄次标准试虫。Small brown planthoppers（Laodelphgax striatellus） are agricultural pests and mediators to spreadplant virus.In order to provide sufficient insect materials in vivo for correlational research,a method for massrearing of small brown planthopper in laboratory was presented.We optimized the temperature,light intensity,rearing density and fixed the time of laying eggs,changing seedlings through a series of biological tests.Resultsshowed that temperature of 24-28℃,light intensity of 5000-7000 lx,rearing density of 300-400 SBPH perbeaker（nymph）,200-300 SBPH per beaker（3th-4th instar）,and 100-200 SBPH per beaker（adults） were thebest condition for rice growing,SBPH development,breeding and hatching eggs of each rearing batch.For theconsistency of instars between different batches,the times of 48 hours and 8 to 10 days were respectively fixedfor laying eggs and changing seedlings.Factors of rearing condition were explicit using this method,and wereeasy to operate.It was also not limited to space,time,environment and other natural conditions and couldprovide enough insects at standard experimental age at any time.

关 键 词：灰飞虱 水稻条纹叶枯病 水稻黑条矮缩病 玉米粗缩病 饲养方法 

分 类 号：S435.111.49[农业科学—农业昆虫与害虫防治]