异氟醚对老年大鼠空间学习记忆能力及海马RhoA蛋白表达的影响  

Affect of Isoflurane on spatial memory function and hippocampal RhoA expression in aged rats

作　　者：张文超[1] 王庚[1] 胡中华[2] 段开明[2] 欧阳文[2] 

机构地区：[1]北京积水潭医院麻醉科,北京100035 [2]中南大学湘雅三医院麻醉科,湖南长沙410083

出　　处：《中国医药导报》2015年第16期38-41,共4页China Medical Herald

摘　　要：目的探讨异氟醚吸入麻醉对老年大鼠海马CA3区Rho A蛋白表达及空间学习记忆能力的影响。方法筛选出30只健康22月龄SD大鼠,随机分为对照组C组(n=10)、麻醉组Ⅰ组(n=10)和Ⅱ组(n=10)。麻醉组以3%的异氟醚诱导,采用经口明视气管插管建立老年大鼠吸入麻醉模型,并以2%异氟醚维持麻醉2 h。C组大鼠不给任何麻醉药,只置于麻醉箱中吸入氧气2 h,氧流量为2 L/min。Ⅰ、Ⅱ组分别于麻醉后24、72 h进行旷场实验和Y-迷宫实验并计算成绩。比较三组大鼠学习记忆能力,并采用免疫组织化学法检测三组海马区Rho A蛋白表达。结果 1Y-迷宫实验:Ⅰ组的Y-迷宫学习次数[(70.50±16.25)次]明显多于Ⅱ组[(61.80±23.49)次]和C组[(49.20±8.56)次](P<0.05),C组与Ⅱ组比较差异无统计学意义(P>0.05)。旷场实验:三组的中央格停留时间、直立次数、跨格次数、总活动量次数比较,差异无统计学意义(P>0.05)。2免疫组织化学法:Ⅰ组海马CA3区Rho A阳性细胞计数(112.66±11.56)多于Ⅱ组(97.77±7.47)与C组(99.33±12.10)(P<0.05),Ⅱ组与C组比较差异无统计学意义(P>0.05)。结论 22月龄SD大鼠接受异氟醚麻醉后24 h空间学习记忆能力下降,于麻醉后72 h基本恢复,异氟醚麻醉后大鼠海马区Rho蛋白家族信号传导分子变化介导的神经突触形态学改变可能是短期空间学习记忆能力损伤的细胞学基础。Objective To study affect of Isoflurane with inhalation anesthesia on hippocampal RhoA expression and spatial memory function in aged rats. Methods Thirty 22-month-old Sprague-Dawley rats were randomly assigned to control group （group C, 10 cases）, anesthesia group l （10 cases） and anesthesia group Ⅱ （10 cases）. The inhalation anesthesia model of aged rat was established by using endotracheal intubation, 2% Isoflurane was applied to maintain anesthesia of 2 hours after anesthesia induction with 3% Isoflurane by inhalation in anesthesia groups. Any drug was not used in rat of group C, which inhaled 100% oxygen for 2 hours, oxygen flow rate was 2 L/min. Open-field experiment and Y-maze experiment were implemented, performance was calculated after anesthesia of 24, 72 hours in group Ⅰ and group Ⅱ. Learning and memory ability in rats of three groups were compared. Hippocampal RhoA expression in rats of three groups were detected by using the immune histochemical method. Results （1）Y-maze experiment： Y-maze learning time of group I [（70.50±16.25） times] was significantly more than that of group Ⅱ [（61.80±23.49） times] and group C [（49.20±8.56） times] respectively （P 〈 0.05）. Y-maze learning time of group C and group II was compared, with no statistical difference （P 〉 0.05）. Open-field experiment： there were no statistical difference on the middle of residence time, number of uptight, number of across grid, number Of total activity among three groups （P 〉 0.05）. （2）Immune histochemi- cal method： RhoA positive cell count of hippocampal CA3 area in group I （112.66±11.56） was more than that in group Ⅱ （97.77±7.47） and group C （99.33±12.10） respectively （P 〈 0.05）. RhoA positive cell count of hippocampal CA3 area in group Ⅱ and group C was compared, with no statistical difference （P 〉 0.05）. Conclusion Spatial learning and memory ability of 22-month Sprague-Dawley rats received Isoflurane anesthesia of 24 hours decline, basi

关 键 词：异氟醚 Y-迷宫 老年大鼠 RHOGTPASES RHOA 

分 类 号：R332[医药卫生—人体生理学]