HPLC-PDA检测祛痘除螨类化妆品中非法添加盐酸米诺环素方法的建立  

Detection of minocycline hydrochloride illegally added in acne removal cosmetic by HPLC-PDA

作　　者：窦佳 董莉 栾爽 沈丹丹 李晓娜[2] 

机构地区：[1]辽宁省大连市药品检验所,116021 [2]大连理工大学环境学院

出　　处：《职业与健康》2015年第7期906-908,共3页Occupation and Health

基　　金：国家自然科学基金资助(项目编号:21407019)

摘　　要：目的建立用高效液相色谱-二极管阵列检测法（HPLC-PDA）检测祛痘除螨类化妆品中非法添加的盐酸米诺环素,以打击化妆品中非法添加禁限物质的不法行为。方法采用Waters Symmetry C18（5μm,4.6 mm×250 mm）色谱柱;以醋酸铵缓冲盐溶液（取醋酸铵15 g、氯化钾10 g与乙二胺四乙酸二钠5 g,加水溶解并稀释成1 000 ml）-乙腈（70∶30）为流动相等度洗脱;流速为1.0 ml/min;柱温为25℃;检测波长280 nm;用PDA检测,以保留时间和紫外光谱图定性,峰面积定量。结果盐酸米诺环素在1~100μg/ml范围内与峰面积呈良好的线性关系,相关系数为1.000 0,检出限为0.1μg/ml,方法的回收率为88.5%~98.5%,RSD为1.0%~1.5%（n=6）。结论该方法特异性强,灵敏度高,准确可靠,适用于不同基质样品中盐酸米诺环素的检测。[Objective]To establish a HPLC-PDA method for detecting the minocycline hydrochloride illegally added in acne removal cosmetics, crack down the delict of illegally adding the prohibited and restricted substances in the cosmetics. [Methods] The separation was performed on a Waters Symmetry C18（5 μm, 4.6 mm×250 mm） column using ammonium acetate buffer solution （dissolve 15 g ammonium acetate, 10 g potassium chloride and 5 g EDTA-2Na in water,and dilute to 1 000 ml）- acetonitrile（70：30） as mobile phase with the detection wavelength at 280 nm, flow rate of 1.0 ml/min and column temperature 25 ℃. PDA was used for detecting, retention time and UV spectrum were used for qualitative analysis, and peak area was used for quantification. [Results]The calibration curve of minocycline hydroehloride showed good linearity within the range of 1-100 μg/ml （r=1.000 0）. The detection limit was 0.1 μg/ml. The recovery rate was 88.5%-98.5%, the RSD was 1.0%-1.5%（n=6）. [ Conclusion]This method is specific, sensitive, accurate and reliable, which is applicable for the detection of minoeycline hydrochlofide in different matrix samples.

关 键 词：祛痘除螨类化妆品 盐酸米诺环素 高效液相色谱-二极管阵列检测法 

分 类 号：R134.2[医药卫生—劳动卫生]