DHPLC和PCR-RFLP技术筛查下肢静脉血栓患者EPCR多态基因  

Screening EPCR gene polymorphism on patients of lower extremity venous thrombosis with Denaturing HPLC and PCR-RFLP technology

作　　者：孙新六[1] 阴冠程[1] 

机构地区：[1]泰山医学院附属泰山医院,山东泰安271000

出　　处：《泰山医学院学报》2015年第2期137-139,共3页Journal of Taishan Medical College

摘　　要：目的探讨下肢静脉血栓患者内皮细胞蛋白C受体基因(EPCR)多态性分布状态。方法应用酶联免疫吸附试验(ELISA)检测血浆s EPCR水平;采用变性高效液相色谱技术(d HPLC)和限制性片段长度多态性聚合酶链反应(PCR-RFLP)技术检测内皮细胞蛋白C受体基因6936位点单核苷酸(SNP)多态性。结果在下肢静脉血栓组,G等位基因的频率(24.0%)和血浆s EPCR平均水平(141.6+30.1 ng/ml),显著高于对照组G等位基因频率(9.4%)和s EPCR平均水平(110.2+26.4 ng/ml)。结论 EPCR6936的基因多态性与下肢静脉血栓风险相关。Objective：To discussing endothelial cell protein C receptor gene（EPC）polymorphism distribution state On patients with lower extremity venous thrombosis. Methods：The plasma soluble endothelial cell protein C receptor（sEPCR） levels were measured by enzyme - linked immunosorbent assay（ELISA）method;Analysed endothelial cell protein C recep-tor gene SNP with denaturing high - performance liquid chromatography（ DHPLC）and Polymerase chain reaction - restric-tion fragment length polymorphism（ PCR - RFLP）. Results：In lower extremities thrombosis group,G allele frequency （24. 0% ）and plasma sEPCR mean levels（141. 6 ＋ 30. 1 ng/ ml）is significantly higher than control group（9. 4% for G allele frequency and 110. 2 ＋ 26. 4 ng/ ml for sEPCR mean levels）. Conclusions EPCR6936 gene polymorphism is associat-ed with the risk of lower extremity venous thrombosis.

关 键 词：内皮细胞蛋白 C 受体 多态性 下肢静脉血栓 

分 类 号：R543.6[医药卫生—心血管疾病]