Identification of differentially-expressed genes of rice in overlapping responses to bacterial infection by Xanthomonas oryzae pv.oryzae and nitrogen deficiency  

作　　者：YU Chao CHEN Hua-min TIAN Fang BI Yong-mei Rothstein J Steven Leach E Jan HE Chen-yang 

机构地区：[1]State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences [2]Department of Molecular and Cellular Biology,College of Biological Science,University of Guelph [3]Department of Bioagricultural Sciences and Pest Management,Colorado State University

出　　处：《Journal of Integrative Agriculture》2015年第5期888-899,共12页农业科学学报（英文版）

基　　金：supported by the grants from the National Basic Research Program of China(2011CB100701)

摘　　要：Bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae（Xoo） is one of high nitrogen（N） responsive diseases.Rice plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might exist.However,the co-regulatory components in such crosstalk have not been elucidated.Here,we comparatively analyzed the gene expression profiling of rice under Xoo inoculation,low N treatment,or a combination of both stresses,and identified the differentially-expressed genes（DEGs） in overlapping responses.These DEGs were involved in different biological processes,including innate immunity and nitrogen metabolism.The randomly-selected DEGs expression was validated by quantitative real-time PCR assays.Temporal expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were present.These DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.Bacterial blight of rice caused by Xanthomonas oryzae pv.oryzae（Xoo） is one of high nitrogen（N） responsive diseases.Rice plants became more disease resistant with decreasing N suggesting that the crosstalk between disease resistance and N utilization pathways might exist.However,the co-regulatory components in such crosstalk have not been elucidated.Here,we comparatively analyzed the gene expression profiling of rice under Xoo inoculation,low N treatment,or a combination of both stresses,and identified the differentially-expressed genes（DEGs） in overlapping responses.These DEGs were involved in different biological processes,including innate immunity and nitrogen metabolism.The randomly-selected DEGs expression was validated by quantitative real-time PCR assays.Temporal expression of six genes from different functional categories suggested that N condition was the dominant factor when both stresses were present.These DEGs identified provide novel insights into the coordinated regulatory mechanism in biotic and abiotic stress responses in rice.

关 键 词：Xanthomonas differentially overlapping abiotic blight responsive crosstalk pathogen encoding validated 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治] Q786[农业科学—植物保护]