山羊角药材的HPLC指纹图谱研究  

Study on HPLC Fingerprint of Capra hireus Linnaeus

作　　者：康馨元[1] 刘睿[2] 李春楠[1] 钱大玮[2] 孙佳明[1] 段金廒[2] 张辉[1] 

机构地区：[1]长春中医药大学,吉林长春130117 [2]南京中医药大学,江苏南京210046

出　　处：《中国现代中药》2015年第5期453-457,共5页Modern Chinese Medicine

基　　金：角类动物药的标准制定项目--国家药品标准提高暨2015版药典科研任务(2012)

摘　　要：目的:建立山羊角药材的HPLC指纹图谱。方法:采用高效液相色谱法,色谱柱为Venusil ASB C18(250 mm×4.6 mm,5μm);流动相为0.1%甲酸水-乙腈,梯度洗脱;检测波长为254 nm;流速为1.0 m L·min-1。结果:建立了10个不同产地山羊角药材的共有图谱,确定了10个共有峰,采用液质联用技术指认了其中4个共有峰,并采用聚类分析、主成分分析、相似度分析的方法对指纹图谱进行了分析。结论:该方法简便快速、专属性强,可用于山羊角药材的鉴别和评价。Objective： To set u Pthe HPLC fingerprint for Capra hireus Linnaeus. Methods： The separation was performed by HPLC. Venusil ASB C18 （4. 6 ×250 mm, 5 μm）column was used with mixture of 0. 1% formic acid-water and acetonitrile as mobile phase in gradient mode. The detection wavelength was 254 nm. The flow rate was 1.0 mL ·min^-1. Results： The HPLC fingerprint for Capra hireus Linnaeus collected from ten different sources was set up. There were ten common peaks, four of witch had been identified by HPLC-DAD-ESI/MS/MS. The fingerprint was analyzed with methods of cluster analysis, principal component analysis and similarity computation. Conclusion： The method is simple, rapid and can be used for identification and evaluation of Capra hireus Linnaeus.

关 键 词：山羊角 高效液相色谱法 指纹图谱 

分 类 号：R944[医药卫生—药剂学]