妊娠期糖尿病小鼠胎盘EGFR的表达与其发病关系的研究  

Relationship between Expression of EGFR in Placenta of GDM Mice and Gestational Diabetes Mellitus

作　　者：张梅[1] 王宏兰[2] 张坤 刘秀财[1] 郭红艳[1] 李淑艳[1] 

机构地区：[1]齐齐哈尔医学院生物化学教研室,黑龙江齐齐哈尔161042 [2]齐齐哈尔医学院临床生化教研室,黑龙江齐齐哈尔161042 [3]佳木斯出入境检验检疫局,黑龙江佳木斯154000

出　　处：《现代生物医学进展》2015年第17期3232-3235,3289,共5页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12521643)

摘　　要：目的:研究妊娠期糖尿病小鼠胎盘表皮生长因子受体(EGFR)的表达,探讨EGFR表达与妊娠期糖尿病发病的关系。方法:采用链脲佐菌素建立妊娠期糖尿病(gestational diabetes mellitus,GDM)小鼠模型,对照组为正常妊娠小鼠,腹腔注射等量缓冲溶液。测定母鼠体重、血糖;计算胎鼠的存活率;测定胎鼠、胎盘重量,计算胎盘效率;RT-PCR、免疫组化分别测定GDM组和对照组胎盘EGFR m RNA和EGFR蛋白的表达。Pearson相关性分析用于母鼠血糖与EGFR表达的相关性分析。结果:GDM组母鼠体重和血糖均高于对照组(P<0.01);GDM组胎鼠、胎盘重量及胎盘效率均高于对照组(P<0.01);RT-PCR和免疫组化结果显示GDM组胎盘EGFR m RNA和EGFR蛋白的表达与对照组相比差异有统计学意义(P<0.01)。GDM组小鼠血糖值与其胎盘EGFR的表达具有相关性(r=0.582,P<0.05)。结论:GDM导致胎盘EGFR表达升高,EGFR并不是GDM的发病因素,EGFR是预防GDM的潜在靶点。Objective： To study the relationship between the expression of EGFR in placenta of gestational diabetes mellitus（GDM） mice and gestational diabetes mellitus. Methods： Streptozotocin was used to induce GDM in mice, the control group（normal pregnancy） was given the same dose buffer solution. mother＇s blood glucose and body weight was obtained. The weight of fetal mice and placenta were determined after birth, then placental efficiency was measured immediately. The expression of EGFR m RNA and protein in placenta were determined by RT-PCR and immunohistochemistry. The relationship between expression of EGFR and mother＇s blood glucose were analyzed by Pearson correlation analysis. Results： Mother＇s blood glucose and body weight in GDM group were higher than that of control group（P〈0.01）; The weight of fetal mice and placenta and placental efficiency in GDM group were significantly higher than that of control group（P〈0.01）. The expression of EGFR m RNA and protein had significant difference between GDM group and control group（P〈0.01）, with statistical significance. There was a correlation between mother＇s blood glucose and the expression of EGFR（r=0.582, P〈0.05）. Conclusions： GDM is the reason of increased expression of EGFR in placenta, but EGFR is not pathogenetic factors of GDM. EGFR is the potential target for prevention.

关 键 词：妊娠期糖尿病 表皮生长因子 表皮生长因子受体 

分 类 号：Q95-3[生物学—动物学] R714.256[医药卫生—妇产科学]