GLT-1在高压氧致中枢神经系统氧中毒发生中的作用研究  

Effects of Glutamate Transporter GLT-1 in Central Nervous System Oxygen Toxicity Caused by Hyperbaric Oxygen Exposure

作　　者：陈宇亮 张亚楠[2] 张婷[2] 徐伟刚[2] 李润平[2] 

机构地区：[1]北京市海军总医院航海航空医学中心,北京100048 [2]上海第二军医大学潜水医学教研室,上海200433

出　　处：《现代生物医学进展》2015年第18期3422-3425,共4页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81272179);第二军医大学军事医学专项课题(2011JS02)

摘　　要：目的:探讨谷氨酸及其转运体在高压氧(hyperbaric oxygen,HBO)致中枢神经系统氧中毒(central nervous system oxygen toxicity,CNS-OT)发生中的作用。方法:(1)大鼠侧脑室注射谷氨酸转运体(Glutamate Transporter-1,GLT-1)选择性激动剂Ceftriaxone后观察氧惊厥潜伏期。采用随机数字法将大鼠分为对照组和50μg、100μg以及200μg Ceftriaxone给药组。采用侧脑室注射方法分别给予生理盐水和不同剂量Ceftriaxone后,进行0.6 MPa HBO暴露,记录大鼠的惊厥潜伏期。(2)大鼠侧脑室注射GLT-1选择性抑制剂DHK后观察氧惊厥潜伏期。采用随机数字法将大鼠分为对照组和5μg、10μg以及20μg DHK给药组。采用脑室注射方法分别给予生理盐水和不同剂量DHK后,进行0.6 MPa HBO暴露,记录大鼠的惊厥潜伏期。结果:脑室注射100μg Ceftriaxone组(33分4.2秒4分12.4秒)和200μg Ceftriaxone组(47分4.2秒5分5.2秒)惊厥潜伏期显著延长,差异有统计学意义(P 0.05),并存在一定量效关系。脑室注射5μg DHK组(16分44.4秒±2分4.7秒)、10μg DHK组(12分51秒±1分23.3秒)和20μg DHK组(7分31.1秒±53秒)惊厥潜伏期显著缩短,差异有统计学意义(P 0.05),并存在一定量效关系。结论:中枢局部给予GLT-1激动剂可以有效延长CNS-OT的潜伏期;中枢局部给予GLT-1抑制剂可以有效缩短CNS-OT的潜伏期。Objective： To investigate the effects of glutamate and glutamate transporter（GLT-1） in central nervous system oxygen toxicity caused by hyperbaric oxygen exposure. Methods： 1. The changes of the latency of central nervous system oxygen toxicity after intracerebroventricularly injecting GLT-1 agonist Ceftriaxone. 24 sprague-dawley rats were randomly divided into 4 groups, each consisting of 6 animals： the saline group（the animals were intracerebroventricularly injected 20 μL saline）, 50 μg Ceftriaxone group（the animals were intracerebroventricularly injected 50 μg Ceftriaxone）, 100 μg Ceftriaxone group（the animals were intracerebroventricularly injected 100 μg Ceftriaxone）, 200 μg Ceftriaxone group（the animals were intracerebroventricularly injected 200 μg Ceftriaxone）. The latency of central nervous system oxygen toxicity was observed and recorded during the exposure of 6 ATA hyperbaric oxygen. 2. The changes of the latency of central nervous system oxygen toxicity after intracerebroventricularly injecting GLT-1 antagonist DHK. 24sprague-dawley rats were randomly divided into 4 groups, each consisting of 6 animals： the saline group（the animals were intracerebroventricularly injected 20 μL saline）, 5 μg DHK group（the animals were intracerebroventricularly injected 5 μg DHK）, 10 μg DHK group（the animals were intracerebroventricularly injected 10 μg DHK）, and 20 μg DHK group（the animals were intracerebroventricularly injected 20 μg DHK）. The latency of central nervous system oxygen toxicity was observed and recorded during the exposure of 6 ATA hyperbaric oxygen. Results： The latency of central nervous system oxygen toxicity of 100 μg Ceftriaxone group（33 min 4.2 sec 4 min 12.4 sec） and 200 μg Ceftriaxone group（47 min 4.2 sec 5 min 5.2 sec） were significantly longer than that of saline group and there was dose-response relationship between the two groups. The latency of central nervous system oxygen toxicity of 5 μg DHK group（16 min 44.4 sec 2

关 键 词：高压氧 中枢神经系统氧中毒 谷氨酸 谷氨酸转运体 

分 类 号：Q95-3[生物学—动物学] R595.1[医药卫生—内科学]