贵州省2012年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分型鉴定分析  

Typing and identification of non-polio enterovirus from acute flaccid paralysis csaes in Guizhou province,2012

作　　者：王宇[1,2] 任刚[1] 苏飞[1] 唐小敏[1] 王涛[1] 叶绪芳[1] 张丽[1] 朱青[1] 

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004 [2]贵阳医学院寄生虫学教研室

出　　处：《疾病监测与控制》2015年第5期289-291,共3页Journal of Diseases Monitor and Control

基　　金：贵州省疾病预防控制中心青年基金项目(2013-E1-2青)

摘　　要：目的运用分子生物学检测方法对从贵州省2012年AFP(Acute Flaccid Paralysis)病例中分离到的30株非脊灰肠道病毒进行分型鉴定。方法采用简并引物RT-PCR对分离株进行VPl区基因扩增和序列测定,测序结果进行BLAST比较和基因进化分析。结果序列分析结果表明,30株NPEV中,有4株无法扩增VP1基因,另外26株共包括11个血清型。包括EV71、ECHO6、COXA4、EV96、ECHO14、COXA10、ECHO25、ECHO13、ECHO11、COXB5、COXB4。其中EV71有6株,所占比例最高。ECHO6、COXA4各3株,EV96、ECHO14、COXA10、ECHO25、ECHO13、ECHO11各2株,COXB5、COXB4各1株。结论贵州省2012年AFP病例的病原谱可能包括多型别的NPEV。Obiective The 30 strains of the acute flaccid paralysis（AFP） associated non-polio enterovirus（NPEV） isolated from Guizhou provice in 2012 were typed by molecular biological methods. Methods Partial sequencing of the VP1 region was amplified by RT-PCR with degenerate primers and sequenced while sequences were compared with the datbase of Gen Bank by the BLAST algorithm.Evolution was analyzed by constructing phylogenetic tree using Mega4.0. Results In this study, a total of 40 NPEVs were analyzed,including 4 strainsun-typed, 26 strains typed by RT-PCR.A total of 11 serotypes were identified.such as EV71、ECHO6、COXA4、EV96、ECHO14、COXA10、ECHO25、ECHO13、ECHO11、COXB5、COXB4 In the 26 strains,EV71 was the most frequently seen isolates,followed by ECHO6 and COXA4. Conclusion In 2012, the pathogen spectrum of acute flaccid paralysis cases included several serotype of HEV in Guizhou province.

关 键 词：非脊髓灰质炎肠道病毒 AFP病例 分子生物学定型 

分 类 号：R512.4[医药卫生—内科学]