聚丙烯酸功能化LaF_3:Ce^(3+)/Tb^(3+)荧光探针测定鲑鱼精DNA  

Poly( acrylic acid)-functionalized LaF_3:Ce^(3+)/ Tb^(3+) fluorescent probe for hs-DNA detection

作　　者：张艺[1] 李紫薇[1] 高红艳[1] 

机构地区：[1]伊犁师范学院化学与生物科学学院新疆凝聚态相变与微结构实验室,新疆伊宁835000

出　　处：《应用化工》2015年第5期956-958,962,共4页Applied Chemical Industry

基　　金：新疆维吾尔自治区高等学校科研计划项目资助(XJEDU2013S40)

摘　　要：用溶剂热法,160℃条件下,反应12 h,成功制备了La F3:Ce3+/Tb3+晶体(PDF 72-1435)。用XRD对材料进行了表征。并用聚丙烯酸(PAA)对材料进行了功能化,红外吸收光谱显示材料表面成功修饰上羧基(─COOH)。以COOH-La F3:Ce3+/Tb3+材料为荧光探针,基于DNA对材料的发光淬灭,实现了对DNA的定量测定。在p H=5.5条件下,功能化荧光材料的荧光强度和鲑鱼精DNA(hs-DNA)的浓度(0.60~25.0μg/m L)呈线性关系,线性方程为ΔF=113.9-1.5c,检测限为0.97μg/m L,相关系数R=0.998 2,RSD为0.97%。The LaFa ： Ce3＋/Tb3＋ crystals （ PDF 72-1435 ） were prepared using solvothermal method, at 160 ℃ for 12 h. The as-prepared LaF3：Ce3＋/Tb3＋ crystals were investigated by X-ray diffraction and functionlized with poly（ acrylic acid）. IR absorption spectra showed that the carboxyl group （--COOH） is on the surface of LaF3 ：Ce3＋/Tb3＋ crystals. It was found that DNA could quench effectively the lumines- cence of the particles. Based on the luminescence quench, the method was quantitative relation between the amount of nucleic acid. Under pH = 5.5 condition, the fluorescence intensity of functionalized material （--COOH-LaF3 ： Ce3 ＋/Tb3 ＋ ） was proportional to the concentration over the range of 0.60 - 25.0 μg/mL for herring sperm DNA （hs-DNA） , the linear regression equation was AF = 113.9 - 1.5c, the correspond- ing detection limit is 0.97 μg/mL,R =0. 998 2,RSD is 0.97%.

关 键 词：鲑鱼精DNA(hs-DNA) 聚丙烯酸 荧光淬灭 溶剂热法 

分 类 号：TQ133[化学工程—无机化工]