基于极性导向下的龙胆与坚龙胆HPLC特征图谱研究  

HPLC Characteristic Fingerprints of Gentiana scabra Bge. and Gentiana rigescens Franch. Based on the Polarity Orientation Technology

作　　者：玄敏 程雪梅[1,2] 吴靳荣[1,3] 吴立宏[1,2] 王峥涛[1,2] 王长虹[1,2] 

机构地区：[1]上海中医药大学中药研究所中药标准化教育部重点实验室,上海201203 [2]上海中药标准化研究中心,上海201203 [3]上海中医药大学中药学院,上海201203

出　　处：《中国药学杂志》2015年第10期888-893,共6页Chinese Pharmaceutical Journal

基　　金：国家药品标准提高暨2015版药典科研任务(特征图谱在中药材和饮片及中成药质量控制中的应用)(2012)资助

摘　　要：目的建立龙胆与坚龙胆的HPLC特征图谱。方法基于极性导向分离和富集技术,建立龙胆与坚龙胆的HPLC特征图谱。通过相似度分析、聚类分析、主成分分析对特征图谱进行评价。结果龙胆或坚龙胆先经水提取,再经乙酸乙酯萃取,乙酸乙酯萃取部位的HPLC指纹图谱具有明显的特征性。21批龙胆、坚龙胆整体相似度为0.29~0.95;除S4外,6批龙胆的整体相似度为0.82~0.99;14批坚龙胆整体相似度为0.75~0.99,其中10批相似度大于0.90。聚类分析中,样品聚为3类：S4、龙胆、坚龙胆。主成分分析中,21批次的龙胆和坚龙胆样品清晰地分为两类,其中S4与其余批次龙胆样品相距较远。结论所建立特征图谱能够有效鉴别龙胆与坚龙胆,可用于龙胆药材和饮片的质量控制。OBJECTIVE To establish the HPLC characteristic fingerprints of Gentiana scabra Bge. and G. rigescens Franch.METHODS The characteristic fingerprints were established based on polarity orientation technology. Similarity analysis,cluster analysis,and principal component analysis were used to evaluate the fingerprints. RESULTS The HPLC fingerprints of ethyl acetate fraction from the water extract of G. scabra and G. rigescens showed obvious characteristics. The overall similarity of 21 batches of samples was 0. 29- 0. 95. The overall similarity of G. scabra was 0. 82- 0. 99 except S4. And that of G. rigescens was 0. 75- 0. 99 among which 10 batches were greater than 0. 90. In the cluster analysis,the samples were divided into three clusters： S4,G. scabra,and G.rigescens. In the principal component analysis,21 batches of G. scabra. and G. rigescens could be clearly divided into two categories,while S4 was far away from the other samples of G. scabra. CONCLUSION G. scabra and G. rigescens can be identified effectively by the characteristic fingerprints. The method can be used for the quality control of Radix Gentianae.

关 键 词：龙胆 坚龙胆 高效液相色谱法 特征图谱 特征峰 

分 类 号：R284[医药卫生—中药学]