环孢素A对小鼠滋养细胞FasL基因表达及胚胎发育的影响  

Effects of cyclosporine A on the expression of FasL mRNA in mice trophoblast cells and embryonic development

作　　者：吴若愚[1] 刘嘉慧[1] 鲁寒寒 张金香[1] 侯越[1] 李真[2] 袁红花[3] 

机构地区：[1]徐州医学院临床医学系,江苏徐州221004 [2]徐州医学院医学影像学院 [3]徐州医学院神经生物研究中心

出　　处：《徐州医学院学报》2015年第4期216-220,共5页Acta Academiae Medicinae Xuzhou

基　　金：江苏省大学生实践创新训练计划（201310313066x）

摘　　要：目的探讨滋养细胞Fas／FasL基因表达异常、外周T细胞亚群变化对胚胎发育的影响以及环孢素A（CsA）在其中的作用。方法雌性CBA／J小鼠、雄性DBA／2小鼠建立妊娠模型。分别在孕第5天开始经阴道灌注给予不同剂量的CsA乙醇溶液（1mg／kg组、5mg／kg组、25mg／kg组），每日1次直至分娩；RT—PCR法检测小鼠绒毛组织滋养细胞FasL和caspase-3基因表达。部分成模小鼠分别于CsA给药7、14天，流式细胞术检测其肝组织匀浆内CD4^＋CD25^＋T细胞相对含量。结果3组CsA干预孕鼠胚胎吸收率分别为16．99％、13．42％及16．67％，与对照组（18．42％）比较，差异有统计学意义（P〈0．05）；FasL和caspase-3基因表达被抑制，与对照组比较差异有差异有统计学意义（P〈0．05）。CsA干预组CD4^＋CD25^＋T细胞水平明显增加，与自然流产组比较差异有统计学意义（P〈0．05）。结论CsA可抑制FasL以及caspase-3基因的表达，使小鼠CD4^＋CD25^＋T细胞增多。在一定范围内，CsA剂量越高，抑制作用越强，从而维持正常妊娠，减少流产率。Objective To observe the effects of cyclosporine A （CsA） at different concentrations on the quantities of Fas/FasL mRNA in mice trophoblast cells, the number of peripheral T cell subgroups and their potential relationship with embryonic development. Methods A pregnant model was established using CBA/J female rats and DBA/2 male rats. On Day 5 of pregnancy, different concentrations of CsA in ethanol solution （ 1,5 and 25 mg/kg） were perfused into in the vaginas of these rats once per day until delivery. Then the quantities of FasL and caspase - 3 mRNA in mice trophoblast cells were determined by RT - PCR. Meanwhile, some model mice were administrated with CsA for 7 and 14 days to measure the number of CD4 ^＋ CD25^ ＋ T ceils within liver tissue homogenates through flow cytometry. Results The rate of embryo resorption was 16.99% for Group 1 mg/kg, 13.42% for Group 5 mk/kg, and 16.67% for Group 25 mg/kg, which was significantly different from that of the control （18.42% , P 〈0.05 ）. The expression of FasL and caspase -3 in three CsA - treated groups was remarkably suppressed at transcriptional level in comparison with the control （ P 〈 0.05）. After CsA intervention, an obvious increase was seen in the number of CD4^ ＋ CD25^ ＋T cells, compared with the natural absorption group. Conclusion CsA can inhibit the expression of FasL and caspase -3 mRNA, resulting in an increased number of CD4 ^＋ CD25 ^＋ T ceils in mice. In a certain range, the inhibitory effects were enhanced along with the rise of the concentration of CsA. Thus, normal pregnancy is maintained and the rate of abortion will be reduced.

关 键 词：FAS FASL caspase-3 环孢素A 胚胎发育 流产 

分 类 号：R392.12[医药卫生—免疫学]