不同胶原蛋白及TGF—β1参与糖尿病心肌纤维化的形成  

Various collagen types and TGF -β1 participate in the development of cardiac fibrosis induced by diabetes

作　　者：陈冰[1] 彭娇娇[1] 蔡启航 高鹏[2] 张帆[2] 朱霞[3] 乔伟丽[1] 

机构地区：[1]徐州医学院生理学教研室,江苏徐州221004 [2]徐州医学院临床医学系 [3]徐州医学院江苏省新药与临床应用重点实验室

出　　处：《徐州医学院学报》2015年第5期281-285,共5页Acta Academiae Medicinae Xuzhou

基　　金：国家自然科学基金（81173104）;江苏省高等学校大学生实践创新训练计划（2012JSSPITP1870,201310313067X）

摘　　要：目的观察1型和2型糖尿病模型鼠心脏胶原蛋白及转化生长因子β1（TGF-β1）的变化。方法采用链脲霉素（STZ）诱导的糖尿病大鼠和db／db糖尿病小鼠建立糖尿病模型。Masson染色方法观察心脏胶原含量及病理学改变，评估心肌纤维化程度；免疫组织化学方法观察心脏Ⅰ型胶原、Ⅲ型胶原、Ⅳ型胶原、层粘蛋白和α-平滑肌肌动蛋白（α—SMA）的变化；免疫组织化学和免疫印迹法检测心脏TGF—β1的改变。结果与正常组相比，STZ糖尿病大鼠和db／db糖尿病小鼠心脏组织中均出现明显的纤维化改变，表现为胶原蛋白过度沉积，胶原面积百分比显著增加，Ⅰ型胶原、Ⅲ型胶原、Ⅳ型胶原、层粘蛋白和α—SMA蛋白表达增加（P〈0．05）。同时，TGF—β1蛋白表达较正常组也明显增多（P〈0．05）。结论糖尿病心肌纤维化进程中多种胶原蛋白和TGF—β1的表达增加。Objective To observe the changes of different collagen types and TGF - β1 in the heart of mice with type Ⅰ/Ⅱ diabetes mellitus. Methods A rat model of diabetic cardiomyopathy was established through injection of streptozotocin, while db/db mice were adopted in the current study. The myocardial pathological changes and myocardial collagen content were detected by Masson staining. The levels of collagen types Ⅰ , Ⅲand Ⅳ, laminin and α - SMA were measured by immunohistochemistry. The quantity of TGF - β1 was determined by immunohistochemistry and Western blotting. Results Compared with the normal group, cardiac fibrosis was obviously seen in STZ - induced diabetic rats and db/db mice, especially excessive deposition of collagen, and remarkable increases in the percentage of collagen area and the amounts of collagen types Ⅰ, m and Ⅳ, laminin and α - SMA （P 〈 0.05）. Meanwhile, both diabetic rats and db/db mice produced higher levels of TGF- β1 than the normal （P 〈 0.05）. Conclusion The increased amounts of various collagen types and TGF - β1 are determined during the development of cardiac fibrosis induced by diabetes.

关 键 词：糖尿病 心肌纤维化 胶原 TGF-Β1 

分 类 号：R331.36[医药卫生—人体生理学]