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作 者:汤保贵[1] 杨思楷 郑素碧 倪芙镕 周星星[1] 徐月容[1] 谢伟莹[1]
出 处:《淡水渔业》2015年第3期102-104,108,共4页Freshwater Fisheries
基 金:广东海洋大学基金项目"麻醉剂对卵形鲳鯵的生理效应及应用研究";湛江市科技计划项目"几种麻醉剂对卵形鲳鲹的麻醉及应用研究"[湛科(2009)66];广东省科技计划项目"现代渔业专业镇产学研创新联盟建设"[2010B080201003]
摘 要:分别用3个浓度梯度的MS-222和苯唑卡因对尼罗罗非鱼(Oreochromis niloticus)(6.050 0±1.710 3)g进行6 h浸泡式麻醉(水温30.7-32.3℃),然后抽取尾部静脉血,测定血液生理生化指标。重点分析了与生物携氧功能密切相关的6个指标的变化,结果显示:(1)较高浓度(25 mg/L、40 mg/L)MS-222导致红细胞数目(Er)升高,而苯唑卡因导致该值大幅下降;(2)两种麻醉剂对红细胞平均血红蛋白含量(MCH)都无显著影响,且MS-222处理后,血液的血红蛋白浓度(Hb)也无明显变化,但经苯唑卡因麻醉处理后,Hb显著下降,且降幅随药物浓度升高而显著增大。Oreochromis niloticus with body weight of (6.0500 ±1.7103 ) g was exposed to MS-222 and benzocaine for 6 hrs in 3 concentration gradients ( with water temperature of 30.7-32.3 ℃) , and blood from caudal vein was abstracted to determine the hematological parameters .The results showed that , the erythrocyte count ( Er) increased while exposed to MS-222 in high concentrations (25 mg/L and 40 mg/L), however, decreased dramatically while exposed to benzocaine . The mean corpuscular hemoglobin contents ( MCH) of fish exposed to anesthetics did not changed significantly comparing with the control group, so did the hemoglobin concentration (Hb) of fish exposed to MS-222.However, benzocaine generated marked drop in Hb , furthermore, the drop became greater with the increasing of the anesthetic concentration .
关 键 词:MS-222 苯唑卡因 尼罗罗非鱼(Oreochromis niloticus) 红细胞 血红蛋白
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