中性人乳寡糖的分离及结构鉴定  

Isolation and Structural Identification of Neutral Human Milk Oligosaccharides

作　　者：刘世龙 郎银芝 祝贺 闫立娜 吕友晶[1] 赵小亮[1] 蔡超[1] 于广利[1] 

机构地区：[1]山东省糖科学与糖工程重点实验室海洋药物教育部重点实验室中国海洋大学医药学院,青岛266003

出　　处：《高等学校化学学报》2015年第6期1087-1093,共7页Chemical Journal of Chinese Universities

基　　金：国家科技支撑计划(批准号:2013BAB01B02);国家自然科学基金-山东省政府联合基金(批准号:U1406402)资助~~

摘　　要：以脱脂、除蛋白后的人乳为原料,联合运用阴离子交换色谱(AEC)、凝胶渗透色谱(GPC)和多孔石墨化碳色谱(PGC)等多种分离纯化技术制备了18种中性人乳寡糖,并采用电喷雾碰撞诱导串联质谱(ESI-CIDMS/MS)技术对其结构进行了鉴定.结果表明,所制备的中性人乳寡糖主要为三至九糖,含有半乳糖(Gal)、葡萄糖(Glc)、N-乙酰葡萄糖胺(GlcNAc)和岩藻糖(Fuc).所有寡糖的还原端均含有乳糖核心(Galβ1-4Glc),非还原端均含有乳糖胺(Galβ1-3/4GlcNAc),并且Fuc以α1-2,α1-3和α1-4连接于主链的Gal,Glc和GlcNAc上.Eighteen natural oligosaccharides were obtained from the defatted and deproteinated human milk using a combination isolation process including anion exchange chromatography（ AEC ）, gel permeation chro- matography（GPC） and porous graphitized carbon（PGC） liquid chromatography. Meanwhile, the structures of these oligosaccharides were identified by electrospray ionization collision-induced dissociation tandem mass spectrometry（ESI-CID-MS/MS）. The major building blocks of neutral human milk oligosaccharides were con- sisted of tri- to nonasaccharides through cosamine （GlcNAc） and fucose （ Fuc ）. the regular assembly of glucose （ Glc）, galactose （ Gal）, N-acetylglu- Lactose （Gal/31-4Glc） core was located at the reducing end in all of these oligosaccharides while the lactosamine（Gal/31-3/4GlcNAc） was determined at the non-reducing end. In addition, some of Gal, Glc and GlcNAc residues on the backbone of oligosaccharides were fucosylated in α1-2, α1-3 and α1-4 linkages, respectively.

关 键 词：中性人乳寡糖 分离 电喷雾碰撞诱导串联质谱 

分 类 号：O629.1[理学—有机化学]