丽格海棠组织培养技术研究  

Study on Tissue Culture Technology of Begonia elalior

作　　者：曾佑炜[1] 李进进[1] 吕镇城[2] 

机构地区：[1]广东轻工职业技术学院环境工程系,广东广州510300 [2]惠州学院生命科学系,广东惠州516001

出　　处：《安徽农业科学》2015年第17期20-24,共5页Journal of Anhui Agricultural Sciences

基　　金：广东轻工职业技术学院自然科学基金项目(KJ201312);广东省青年创新人才项目(2014KQNCX214)

摘　　要：[目的]通过组织培养方法,筛选丽格海棠不定芽诱导、继代培养和生根培养的最佳培养基,为丽格海棠的快速繁殖提供参考。[方法]采用丽格海棠叶片、叶柄、茎段为外植体进行不定芽诱导、继代及生根培养。[结果]MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+腺嘌呤8 mg/L是诱导叶片愈伤组织培养的最佳激素配比;MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+腺嘌呤8 mg/L是诱导叶柄的最佳激素配比;MS+6-BA 0.5 mg/L+NAA 0.4 mg/L+2,4-D 0.1 mg/L是诱导茎段愈伤组织培养的最佳激素配比;MS+6-BA 1.0 mg/L+IBA 0.2mg/L是诱导丽格海棠继代培养的最佳激素配比;1/2MS+IBA 0.1 mg/L+NAA 0.1 mg/L是诱导丽格海棠生根培养的最佳激素配比,叶片更适宜应用于组织培养。[结论]为丽格海棠织培养快速繁殖和遗传转化提供理论依据。[ Objective ] The objective of this study was to develop an efficient system for the regeneration of Begonia elalior by investigating the factors influencing callus and shoot induction. [ Method ] Leaves, petioles and stems of Begonia elalior were used as explants for adventitious bud induction, subcuhure and rooting. [ Result] The optimum medium for the differentiation and elongation for leaves and petioles or stems was MS ＋ 6-BA 1.0 mg/L ＋ NAA 0.2 mg/L ＋ adenine 8 mg/L, MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.2 mg/L ＋ adenine 8 mg/L, MS ＋ 6-BA 0.5 mg/L ＋ NAA 0.4 mg/L ＋ 2,4-D 0.1 mg/L respectively ; The optimum medium for the secondary and the rooting culture was MS ＋ 6-BA 1.0 mg/L ＋ IBA 0.2 mg/L and 1/2MS ＋ IBA 0.1 mg/L ＋ NAA 0.1 rag/L, respectively. [ Conclusion] The rapid propagation system of Begonia elalior was established in this study, which could provide theoretical evidence for the rapid propagation and genetic transformation of Begonia elalior.

关 键 词：丽格海棠 组织培养 最佳配比 

分 类 号：S682.29[农业科学—观赏园艺]