黄菠萝嫩茎离体培养再生体系的建立  

Establishment for Regeneration System of Younger Stem of Phellodendron amurense Rupr. in vitro

作　　者：王新天 王新宇 李晓萍 王红艳 王伶俐 郭志新 张成婧 韩学慧 刘欣[3] 

机构地区：[1]双辽市林木种苗管理站,吉林四平136400 [2]双辽市郑家屯街林业站,吉林四平136400 [3]吉林省林业勘察设计研究院,吉林长春130033

出　　处：《安徽农业科学》2015年第17期310-312,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]优化黄菠萝离体培养再生体系。[方法]以黄菠萝带腋芽的嫩茎为外植体,采用正交设计研究其离体培养再生体系。[结果]诱导黄菠萝的最佳初代培养基是MS+1.5 mg/L 6-BA+0.06 mg/L NAA+20 g/L蔗糖,继代培养的最佳培养基组合是MS+1.0mg/L 6-BA+0.1 mg/L NAA,最佳生根培养基组合是1/2MS+0.4 mg/LNAA+30 g/L蔗糖。[结论]以黄菠萝带腋芽的嫩茎为外植体,采用正交设计研究其离体培养和植株再生技术方法是可行的。[ Objective ] To optimize Phellodendron amurense Rupr. in vitro culture regeneration system. [ Method ] By using axillary bud-bearing young stem of Phellodendron amurense as explants, the orthogonal experimental design was employed to study the in vitro culture and regeneration condition. [Result] The optimum primary medium was MS ＋ 1.5 mg/L 6-BA ＋0.06 mg/L NAA ＋20 g/L sucrose, the best grouch regulatory substance combination of medium for subculture was MS ＋ 1.0 mg/L 6-BA ＋0.1 mg/L NAA, and the best rooting medium was 1/2MS ＋0.4 mg/LNAA ＋ 30 g/L sucrose. [ Conclusion] Taking axillary bud -bearing young stem of PheUodendron amurense as explants, adopting orthogonal design to study its in vitro culture and plants regeneration technique is feasible.

关 键 词：黄菠萝 组织培养 再生体系 正交设计 

分 类 号：S604.3[农业科学—园艺学]