表没食子儿茶素没食子酸酯对脂多糖诱导原代胶质细胞炎性反应的保护作用  

Neuroprotective effects of epigallocatechin-3-gallate on LPS-induced inflammation in primary gliacytes

作　　者：王馨[1] 严磊[2] 苗加伟[3] 封玉玲[3] 李晶[3] 

机构地区：[1]重钢总医院药剂科,重庆400080 [2]重庆医科大学动物实验中心,重庆400016 [3]重庆三峡医药高等专科学校基础医学部,重庆404120

出　　处：《中国医院药学杂志》2015年第10期917-920,共4页Chinese Journal of Hospital Pharmacy

基　　金：重庆市卫生局医学科研计划重点项目(编号:20121096);重庆市高等教育教学改革研究项目(编号:132128;133309)

摘　　要：目的:观察表没食子儿茶素没食子酸酯[(–)-Epigallocatechin-3-gallate,EGCG]对细菌脂多糖(LPS)所致原代神经胶质细胞炎性反应的保护作用。方法:取新生乳鼠原代神经胶质细胞培养,利用LPS引起其炎症反应。用酶联免疫吸附试验(ELISA)测定炎性因子肿瘤坏死因子(TNF-α)、白介素-1β(IL-1β)、白介素-1β(IL-8)表达情况和蛋白免疫印记法(Western blot)检测炎症因子(诱导型一氧化氮合酶)i NOS蛋白含量变化。结果:LPS激活神经胶质细胞后诱导炎症因子过度表达,大幅上调TNF-α、IL-1β、IL-8炎性因子(P<0.05),i NOS蛋白质水平显著升高(P<0.05),不同浓度EGCG干预组均可明显抑制炎症因子的过度产生,一定程度上减轻LPS诱导神经胶质细胞的炎性反应。结论:一定浓度范围内,EGCG能减弱LPS引起的体外培养神经胶质细胞的炎症反应。OBJECTIVE To determine effects of EGCG on lipopolysaccharide （LPS）-induced neuroinflammation and further investigate the role of EGCG mediated neuroprotectiorL METHODS Primary rat gliacyte cultures were used as an in vitro mod- el to examine effects of EGCG on LPS-induced neuronal damage. Protein levels of inducible nitric oxide synthase （iNOS）, tumor necrosis factor-alpha （TNF-α）, interleukin-1β （IL-1β） and interleukin-8 （IL-8） were determined by ELISA and western blot. RESULTS Compared with blank control group, LPS apparently induced production of intracellular inflammatory re- sponse, and increased releases of TNFα, IL-1β and IL-8 in supernatant of culture （P〈0. 05）. Compared with LPS group, ECK;G groups significantly reduced release of TNFα, IL-1β and IL-8 （P%0. 05） and level of iNOS protein （P〈0. 05）. CON-CLUSION ECA2G is effective in protecting against LPS-induced neuroinflammation by anti-inflammatory effects.

关 键 词：EGCG 脂多糖 炎症 神经保护作用 

分 类 号：R965[医药卫生—药理学]