中华倒刺鲃仔、稚鱼的耳石微结构与日轮形成特征  

Otolith Microstructure and Daily Increment Formation in Larval and Juvenile Qingbo( Spinibarbus sinensis)

作　　者：李忠利[1] 黄辉[2] 冉辉[1] 梁正其[1] 何勇[2] 

机构地区：[1]铜仁学院生物与农林工程学院,贵州铜仁554300 [2]西南大学荣昌校区水产系,重庆荣昌402460

出　　处：《水生态学杂志》2015年第3期83-88,共6页Journal of Hydroecology

基　　金：贵州省科技厅联合基金项目(黔科合LH字[2014]7482);贵州省教育厅创新人才团队项目(黔教合人才团队字[2012]08号);贵州省普通高等学校工程中心建设项目(黔教合KY[2012]028号)

摘　　要：以中华倒刺鲃（Spinibarbus sinensis）亲鱼为材料,在实验室养殖条件下以人工孵化的丰年虫（Eubranchipus vernalis）为仔、稚鱼饵料,通过已知日龄法观察其耳石的微结构,分析其日轮形成特征。结果表明,仔、稚鱼的微耳石和矢耳石一般由1个中心核和1个耳石原基组成,少数存在多个中心核或原基现象,星耳石中心核和原基区分不明显。微耳石和矢耳石中心核直径分别为（37.73±5.34）μm和（39.78±7.11）μm,耳石原基直径分别为（16.29±3.46）μm和（17.09±3.88）μm。矢耳石和星耳石轮纹清晰度、规律性、周期性和完整性不及微耳石;微耳石第1条日轮在仔鱼出膜后第2天形成,以后每天沉积1轮。30日龄稚鱼微耳石轮纹数（N）与日龄（T）的关系符合直线模型,相关关系式为：N=1.0016T-0.8753（R2=0.9961,P〈0.01,n=197）,线性方程斜率与1无显著性差异（P〉0.05）。在微耳石和矢耳石样本中共观察到孵化标记轮和转移标记轮2种,其中孵化标记轮的出现率分别为78.68%和83.33%,转移标记轮的出现率分别为29.95%和48.98%。60尾33日龄稚鱼微耳石的生长轮宽度变化范围0.522-2.244μm,平均为（1.087±0.231）μm。The analysis of otolith increment deposition has been broadly applied and developed in age validation of larvae and juvenile fishes. The rate of otolith increment deposition,especially the time of the first increment deposited,is indispensable for determining age. In this study,we examined the otolith microstructure and daily increment deposition of laboratory-hatched larvae and juvenile Spinibarbus sinensis of known age,the first generation of wild Spinibarbus sinensis in Wujiang River. The study will supplement the biological information on Spinibarbus sinensis and provide data for artificial breeding as well as for resource surveys of wild populations. In July 2014,the experiment was conducted at the artificial breeding and releasing station at Pengshui Hydropower Station on Wujiang River. The artificially fertilized eggs of wild Spinibarbus sinensis were incubated for 78 h until all the larvae had released from the membrane. The larvae were then placed in an aquarium（ 100 cm × 60 cm × 60 cm） and fed Eubranchipus vernalis from day 4. Ten larvae of Spinibarbus sinensis were sampled at 19 ∶ 00 each day until day 12 and then at 19 ∶ 00 every other day. The development stage and full length of the all specimen were assessed under a stereomicroscope. The left sagitta,asteriscus and lapillus were extracted for increment counting and width measurement. The results show that there was usually one nucleus and one primordium in each lapillus and sagitta,and a few samples presented multiple nuclei or multiple primordia. However,the nuclei and the primordia in asteriscus were difficult to differentiate. The diameters of the nucleus for each lapillus and sagitta were（ 37. 73 ± 5. 34） μm and（ 39. 78 ± 7. 11） μm,and the diameters of primordia were（ 16. 29 ± 3. 46） μm and（ 17. 09 ± 3. 88） μm,respectively. The lapillus deposits displayed high clarity,regularity,periodicity and completeness compared with deposits on the sagitta and asteriscus. Increments deposited on the lapillus daily after ha

关 键 词：中华倒刺鲃 耳石 微结构 日轮特征 

分 类 号：Q174[生物学—水生生物学]