影响水稻纹枯病菌营养体亲和性分化的因子  

The im pact factors on differentiation of som atic com patibility of Rhizoctonia solani AG-1-IA

作　　者：余功明[1,2,3] 史国英[2] 魏源文[3] 岑贞陆[2] 黄思良[4] 胡春锦[2] 黎起秦[1] 

机构地区：[1]广西大学农学院,南宁530004 [2]广西农业科学院微生物研究所,南宁530007 [3]广西作物遗传改良生物技术重点开放实验室,南宁530007 [4]南阳师范学院生命科学与技术学院,南阳473061

出　　处：《植物病理学报》2015年第3期225-231,共7页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30860159;31101122);广西科技基础平台建设基金项目(10-046-11);广西自然科学基金(桂科青0728030;2011GXNSFA018076);广西农科院公益维持项目(桂农科2012GW06)

摘　　要：为了探明影响水稻纹枯病菌营养体亲和性分化的因子,将水稻纹枯病菌菌株cx-2在不同水稻品种继代接种,并在含不同杀菌剂、具不同p H及不同温度条件的PDA平板上继代培养,用对峙法测定继代菌株与原始接种菌株的营养体亲和性。此外,对营养体亲和性分化菌株与原始菌株的AFLP指纹图谱进行比较。结果显示,在供试的30个水稻品种上连续接种4次后,从9个水稻品种中分离出与原始菌株营养体不亲和的菌株。菌株在不同p H值的PDA平板继代培养4次后,在p H偏碱性端(p H 10、p H 11)开始出现营养体亲和性分化的菌株。在不同农药和温度条件下继代培养10次的菌株中没有分离到营养体亲和性分化的菌株。营养体亲和性分化菌株与原始菌株的AFLP指纹图谱没有差异。To clarify the factors influencing differentiation of somatic compatibility in Rhizoctonia solani AG-1-IA causing rice sheath blight,the pathogen strain cx-2 w as consecutively inoculated into different rice varieties and subcultured on PDA plates w ith different fungicides / p Hs or at different temperatures. The comparison of somatic compatibility w as made betw een the recovered / subcultured isolates and their original strain（ cx-2）based on barrage zone formation on the face-to-face cultures. M eanw hile,amplified fragment length polymorphism（ AFLP） w as used to determine genetic difference betw een the recovered / subcultured isolates w ith alterations in somatic compatibility and their original strain（ cx-2）. The results show ed that the isolates w ith alterations in somatic compatibility w ere recovered from 9 out of 30 rice varieties tested after four consecutive inoculations. The subcultured isolates w ith alterations in somatic compatibility w ere also detected after four consecutive transfers on the PDA plates w ith alkaline p H levels 10 and 11. How ever,no subcultured isolates w ith alterations in somatic compatibility w ere found even after ten consecutive transfers on the PDA plates containing different fungicides or at different temperatures. The isolates w ith alterations in somatic compatibility show ed the same AFLP fingerprint patterns w ith their original strain（ cx-2）.

关 键 词：水稻 水稻纹枯病菌 营养体亲和性 AFLP 

分 类 号：S435.621.2[农业科学—农业昆虫与害虫防治]