新疆加工型辣椒细菌性斑点病的发生和病原鉴定  

The occurrence and pathogen identification of bacterial leaf spot of processing pepper in Xinjiang

作　　者：管晶晶[1,2] 李克梅[1,2] 罗明[1,2] 张春竹 何媛[1] 盛强[1,2] 楚金平 张祥林[4] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆自治区高校农林有害生物监测与安全防控重点实验室,乌鲁木齐830052 [3]新疆巴州农业科学研究所,库尔勒841000 [4]新疆出入境检验检疫局,乌鲁木齐830063

出　　处：《植物病理学报》2015年第3期239-247,共9页Acta Phytopathologica Sinica

基　　金：新疆维吾尔自治区科技成果转化专项资金项目(201154106);新疆农业大学产学研联合培养研究生项目(xjaucxy-yjs-20131025)

摘　　要：新疆加工型辣椒主要产区巴音郭楞蒙古自治州发生了一种严重危害辣椒的细菌性病害。从发病辣椒叶片中分离细菌,通过烟草过敏性反应、马铃薯软腐试验和接种辣椒等致病性测定,确定了13个致病菌株,各菌株之间致病力无明显差异。通过菌体形态、培养性状观察、生理生化反应、寄主范围测定,结合16S r DNA和rpo D基因扩增、序列测定和系统发育分析,将病原菌鉴定为丁香假单胞菌丁香致病变种(Pseudomonas syringae pv.syringae)。病原菌人工接种还能侵染番茄、茄子、马铃薯及黄瓜、四季豆、白菜、萝卜、芹菜等植物。P.syringae pv.syringae引起加工型辣椒细菌性斑点病在国内属首次报道。A serious bacterial leaf spot disease w as commonly found on cultivated processing pepper in Bayinguoleng M ongol Autonomous Prefecture of Xinjiang. In order to identify the causal agent of the disease,bacterial strains w ere isolated from the diseased leaves,and the pathogenicity of the isolates w as then confirmed by inoculation of the isolates on pepper seedling,potato soft rot test and the hypersensitive reaction on tobacco.Thirteen pathogenic isolates w ere obtained. Based on morphological, physiological and biochemical characteristics,pathogenicity test,16S rDNA and rpoD gene phylogenetic analysis,the causal pathogen w as identified as Pseudomonas syringae pv. syringae. By artificial inoculation,the isolates could also affect many other plants,such as tomato,eggplant,potato,cucumber,green bean,cabbage,carrot,celeries,etc.. This is the first report of P. syringae pv. syringae causing leaf spot disease on processing pepper in China.

关 键 词：辣椒叶斑病 PSEUDOMONAS SYRINGAE pv.syringae 生理生化特性 分子鉴定 

分 类 号：S436.418[农业科学—农业昆虫与害虫防治]