纳米羧甲基壳聚糖氟化钠涂膜预防龋齿的实验室研究  

Laboratory Research on Nano-carboxymethyl Chitosan Fluoride Varnish in the Prevention of Dental Caries

作　　者：李长春[1] 许晓燕[1] 徐全臣[1] 宋文斌[1] 刘凤芝[1] 刘沂[1] 

机构地区：[1]青岛大学附属医院口腔内科,山东青岛266003

出　　处：《中国药房》2015年第16期2212-2215,共4页China Pharmacy

摘　　要：目的:研究纳米羧甲基壳聚糖氟化钠涂膜(NCCF涂膜)对龋齿的预防作用。方法:以未处理的牙釉质块为空白对照(A1组),采用原子吸收分光光度法测定分别涂布0.15%NCCF涂膜(B1组)、0.15%柯伯脂含氟涂膜(C1组)、0.15%氟化钠溶液(D1组)的牙釉质块在脱矿液中的钙离子溶出量,采用扫描电镜观察牙釉质表面的超微结构。以涂抹无菌生理盐水的羟基磷灰石片为阴性对照(A2组),采用生物膜活菌计数法考察分别涂布0.15%NCCF涂膜(B2组)、0.15%柯伯脂含氟涂膜(C2组)、0.15%氟化钠溶液(D2组)的羟基磷灰石片在人工唾液中培养12、24、48 h对变形链球菌和血链球菌菌落数的影响。结果:与A1、C1、D1组比较,B1组牙釉质块在脱矿液中钙离子溶出量降低,差异具有统计学意义(P<0.05),釉质表面结构基本完整。与A2、C2、D2组比较,B2组羟基磷灰石片上变形链球菌和血链球菌的菌落数均减少,差异均具有统计学意义(P<0.05)。结论:NCCF涂膜对牙釉质脱矿及口腔中变形链球菌、血链球菌的生长和黏附均具有抑制作用。OBJECTIVE： To study the protective effects of Nano-carboxymethyl chitosan fluride varnish （NCCF varnish） on dental caries. METHODS： Untreated enamel blocks were blank control （A1 group）, atomic absorption spectrometry （AAS） was used to determine the calcium leaching amount in enamel block demineralization solution with 0.15% NCCF varnish （B1 group）, 0.15% copal varnish （C1 group） and 0.15% sodium chloride solution （D1 group）. The electron microscopy was used to scan the ultrastructure of the enamel surface. Hydroxyapatite tablets with sterile normal saline were negative control （A2 group）, biofilm via- ble counting method was used to detect the effects of Hydroxyapatite tablets with 0.15% NCCF varnish （B2 group）, 0.15% copal varnish （C2 group） and 0.15% sodium chloride solution （D2 group） on the colonies of Streptococcus mutans and Streptococcus sanguis cultivating in artificial saliva for 12, 24 and 48 h. RESULTS： Compared with A1, C1 and D1 groups, the calcium leach- ing amount in enamel block demineralization solution in B1 group was decreased, with significant difference （P〈0.05）, and the structure of enamel surface was basically intact. Compared with A2, C2 and D2 groups, the colonies of Streptococcus mutans and Streptococcus sanguis in B2 group were decreased, with significant difference （P〈0.05）. CONCLUSIONS： NCCF varnish has in- hibition effects on enamel demineralization and the growth and adhesion of Streptococcus mutans and Streptococcus sanguis in oral.

关 键 词：羧甲基壳聚糖 氟化物涂膜 钙离子溶出量 龋齿 

分 类 号：R965[医药卫生—药理学]