未足月胎膜早破患者外周血中分泌型磷脂酶A2的表达与羊膜腔感染的相关性  

Correlation of Expression of Secretory Phospholipase A2 in Peripheral Blood and Infection of Amniotic Cavity in Preterm Premature Rupture of Membranes

作　　者：周洋[1] 周佳任[1] 徐漫飞[1] 张怡舜[1] 赵岩[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《中国医科大学学报》2015年第6期520-523,共4页Journal of China Medical University

摘　　要：目的研究外周血中分泌型磷脂酶A2(sPLA2)在未足月胎膜早破及羊膜腔感染中的作用及其意义。方法采用实时荧光定量PCR技术检测30例未足月胎膜早破患者(实验组)分娩发动前后、30例未发生胎膜早破的未足月正常妊娠患者(正常对照组)及30例足月胎膜早破患者(足月对照组)分娩发动后外周血中sPLA2 mRNA的表达水平。未足月胎膜早破患者结束妊娠后取胎膜行病理学检查,以确定有无组织学绒毛膜羊膜炎。结果未分娩发动时sPLA2 mRNA的表达水平实验组为1.079±0.746,正常对照组为0.651±0.481,实验组与同孕周正常对照组相比sPLA2 mRNA的表达水平升高,差异有统计学意义(P=0.011)。分娩发动时外周血中sPLA2 mRNA的表达水平实验组为2.439±0.086,足月对照组为2.575±0.036,实验组与足月对照组相比sPLA2 mRNA的表达水平无统计学差异(P=0.787)。实验组中分娩发动时s PLA2的含量与是否患绒毛膜羊膜炎有关(P=0.008)。结论 sPLA2升高可能参与未足月胎膜早破的发病,并与羊膜腔的感染有关。Objective To explore the role and significance of secretory phospholipase A2（s PLA2）in peripheral blood in preterm premature rupture of membranes（p PROM）and infection of amniotic cavity. Methods RT-PCR was used to detect the expression levels of s PLA2 m RNA in peripheral blood of 30 patients with p PROM（experimental group）,30 non-full term normal pregnant patients without p PROM（normal control group）and 30 full term patients with PROM（full-term control group）before and after delivery. Fetal membranes were collected at the time of delivery of patients with p PROM for pathologic examination to determine histological chorioamnionitis（HCA）. Results The expression levels of s PLA2 m RNA in peripheral blood were 1.079±0.746 and 0.651±0.481 in the experimental group and the normal control group before delivery,respectively,indicating that the expression of s PLA2 m RNA was increased in the experimental group compared with the normal control group（P = 0.011）. The expression levels of s PLA2 m RNA in peripheral blood were 2.439±0.086 and 2.575±0.036 in the experimental group and the full-term control group at labor onset,respectively,indicating that there was no statistically significant difference in the level of s PLA2 m RNA in peripheral blood between the experimental group and the full-term control group at labor onset（P = 0.787）. The level of s PLA2 was related to chorioamnionitis in the experimental group at labor onset. Conclusion The increase of s PLA2 may participate in the pathogenesis of preterm premature rupture of membranes and is related with the infection of chorioamnionitis.

关 键 词：胎膜早破 分泌型磷脂酶A2 绒毛膜羊膜炎 分娩发动 

分 类 号：R714.21[医药卫生—妇产科学]