海洋真菌对苯和甲苯的降解诱导黄烷酮的羟基化转化  

Hydroxylated Biotransformation of Flavone by Marine Fungi Induced Degradation of Benzene and Toluene

作　　者：王坤腾 王来友[1] 蓝文健[3] 颜丹凤[2] 李厚金[2] 

机构地区：[1]广东药学院中医药研究院,广州510006 [2]中山大学化学与化学工程学院,广州510275 [3]中山大学药学院,广州510006

出　　处：《应用化学》2015年第6期671-675,共5页Chinese Journal of Applied Chemistry

基　　金：广东省科技计划项目(2012A031100005;2013B021100010;2013B021100012);广东省自然科学基金项目(S2012010010653)资助~~

摘　　要：真菌在各种氧化酶的作用下能将苯环类化合物转化降解,但转化降解过程中诱发的高活性酶的应用仍未见报道。本文研究海洋真菌Pseudallescheria boydii和Trichoderma erinaceum在添加苯和甲苯的培养条件下,诱导黄烷酮向4'-羟基黄烷酮的转化。结果表明,2种真菌对苯和甲苯有强耐受力,其降解过程中诱导的氧化酶对黄烷酮的羟基化具有高度的区域选择性,即发生4'-羟基化反应,而且转化率最高可达到82%,在酶促进的反应领域具有潜在的应用价值。Aromatic hydrocarbons can be degraded by fungi. However, the application of the highly active oxidases induced in the degradation process is still rare. This paper investigated the biotransformation of flavone by marine fungi Pseudallescheria boydii and Trichoderm~ erinaceum cultured in the media containing benzene and toluene. Pseudallescheria boydii and Trichoderma erinaceum show strong tolerance to benzene and toluene. The hydroxylation enzymes activated by the degradation processes of benzene and toluene are responsible for the biotransformation of flavones, which gives the only product 4＇-hydroxy flavones with high regioselectivity. Furthermore, the transformation yield can reach up to 82%. This biotransformation induced by the degradation of benzene and toluene may have potential applications in the field of enzyme-catalyzed reactions.

关 键 词：海洋真菌 苯 甲苯 黄烷酮 生物转化 羟基化 

分 类 号：O625.1[理学—有机化学] Q814.9[理学—化学]