老龄大鼠尿量、离子浓度及皮质醇的变化  

Changes in urine,ion concentration and cortisol in aged rats

作　　者：吴君[1] 韩芸[2] 梁伟玲[1] 荆雪宁[1] 黄萍[3] 

机构地区：[1]山东中医药高等专科学校,山东烟台264199 [2]滨州医学院,山东烟台264003 [3]广州中医药大学,广州510006

出　　处：《中国中医基础医学杂志》2015年第5期515-516,共2页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家自然科学基金资助项目(30873425);山东省高等学校科技计划项目(J12LM52);山东中医药高等专科学校中西医基础重点学科资助

摘　　要：目的:观察老龄大鼠24 h及水负荷后5 h尿量、尿中Na+、K+、Cl-浓度及血清皮质醇(Cort)含量的变化,研究肾虚多尿模型。方法:老年大鼠20只,青年大鼠20只,用代谢笼法测24 h尿量及水负荷5 h尿量,用生化分析仪测尿液中Na+、K+、Cl-浓度,采用ELISA测血中Cort的含量。结果:老龄大鼠5 h、24 h尿量明显增多,尿液中Na+、Cl-浓度明显增加,K+浓度明显降低,血清中Cort的含量明显降低。结论:老龄大鼠尿量增多,且Na+、K+及Cl-排泄异常,血清中Cort的含量明显降低,表明其可作为肾虚多尿模型进行试验研究。Objective：The model of excessive urination due to Kidney deficiency was investigated by observing the 24 h urination,5 h urination after the water load,urinary Na ^＋ .K ^＋ . Cl^- concentration and the content of cortisol in serum was investigated in the aging rat. Methods ：20 aged rats and 20 young rats were chosen. The 5 h urination after the water load in rats and 24 h urination was measured in metabolic cages. The urinary Na ^＋ , K ^＋, Cl^- concentration was measured by biochemistry analyzer. The concentration of Cort was detected by ELISA in the blood of the natural aging rats. Results The 5 h and 24 h urination of aging rats significantly increased. The quantity discharged of Na ^＋.Cl^- significantly decreased and K ＋ increased, the content of Cort significantly decreased too. Conclusion ： Excessive urination, Abnormal excretion of Na^＋ Cl^- 、 K^＋ and the Content decreased significantly in serum,which indicate the aged rats were used as the model of excessive urination due to kidney deficiency for experimental study.

关 键 词：老龄大鼠 尿量 离子浓度 皮质醇 

分 类 号：R285.5[医药卫生—中药学]