球孢白僵菌对扶桑绵粉蚧致病力的测定  

Virulence measurement of Beauveria bassiana strain to Phenacoccus solenopsis Tinsley

作　　者：袁盛勇[1,2] 孔琼[1,2] 沈登荣[1,2] 张宏瑞[3] 陈斌[3] 薛春丽[1,2] 张红艳[1] 丁丽[1] 

机构地区：[1]红河学院生命科学与技术学院,蒙自661199 [2]云南省高校农作物优质高效栽培与安全控制重点实验室,蒙自661199 [3]云南农业大学植物保护学院,昆明650201

出　　处：《华中农业大学学报》2015年第4期27-30,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30860005);公益性行业(农业)科研专项(201103026);红河学院植物保护硕士授权点建设项目

摘　　要：采用点滴法测定了球孢白僵菌MZ041016菌株在2.0×105、2.0×106、2.0×107和2.0×108个/mL4个浓度的分生孢子液对扶桑绵粉蚧(Phenacoccus solenopsis Tinsley)若虫和成虫的致病力。结果表明:球孢白僵菌MZ041016菌株对扶桑绵粉蚧具有很强的致病性,在分生孢子液2.0×108个/mL浓度时对若虫和成虫的致病力最强。扶桑绵粉蚧1龄、2龄、3龄若虫和雌成虫的致死中浓度分别为(2.10±0.06)×106、(2.43±0.06)×106、(3.20±0.06)×106、(3.54±0.06)×106个/mL,致死中时间分别为(4.52±0.17)、(5.34±0.51)、(6.12±0.35)、(6.48±0.32)d。The toxicity bioassay of Beauveria bassiana Vuillemin MZ041016 to Phenacoccus solenopsis Tinsley during four different concentrations on 2.0×10^5,2.0×10^6,2.0×10^7,2.0×10^8 spores per mL was measured by topical application in laboratory,and 1st,2nd,3rd of larvae and female adults of P.solenopsis were vaccinated respectively.The results indicated that the virulence of B.bassiana MZ041016 to P.solenopsis was high.The accumulative mortality was highest in concentration of 2.0×10^8 spores per mL.The median lethal concentration of 1st,2nd,3rd of larvae and adults of the P.solenopsis was（2.10±0.06）×10^6,（2.43±0.06）×10^6,（3.20±0.06）×10^6,（3.54±0.06）×10^6 spores per mL respectively;the median lethal time was（4.52±0.17）,（5.34±0.51）,（6.12±0.35）,（6.48±0.32）d respectively.

关 键 词：球孢白僵菌 扶桑绵粉蚧 致病力 

分 类 号：S482.39[农业科学—农药学]