重金属胁迫对Pseudomon asalcaligenes LH7抗生素抗性的影响  

Effects of heavy metal stress on antibiotics resistance of Pseudomonas alcaligenes LH7

作　　者：温丽华[1] 许燕滨[1] 周艳[1] 阮晶晶[1] 侯毛宇[1] 孙浩[1] 屈毛毛[1] 袁保红[2] 郑莉[1] 

机构地区：[1]广东工业大学环境科学与工程学院,广州510006 [2]广东药学院基础部,广州510006

出　　处：《生态学报》2015年第10期3411-3418,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金资助项目(40801194);中山市科技计划项目(2013A3FC0243)

摘　　要：从广州某养猪场废水处理系统中筛选出1株优势菌Pseudomonas alcaligenes LH7。为了研究重金属胁迫对细菌抗生素抗性响应的影响,采用琼脂稀释法和K-B纸片扩散法,测定了重金属(Cu2+、Zn2+、Cr6+)的最小抑制浓度(MIC),及不同重金属种类和浓度胁迫下,四种抗生素(红霉素、阿莫西林、头孢拉定、四环素)的抑菌圈直径。结果表明:菌体对Cu2+、Zn2+、Cr6+的MIC分别为125、125、100 mg/L,并且具有四环素、阿莫西林、红霉素和头孢拉定多重抗性。重金属与抗生素之间的交互作用对细菌的抗性有显著影响(P<0.05)。重金属和抗生素间的交互作用随重金属种类和浓度的不同而改变,可分为三类:低浓度重金属与抗生素共存时表现为协同抗性,高浓度时则表现为协同杀菌,如Cr6+或Zn2+与红霉素,Cu2+与头孢拉定;低浓度重金属与抗生素共存时表现为协同杀菌,高浓度时则表现为协同抗性,如Cr6+或Zn2+与阿莫西林;只与共存重金属种类相关的抗性组合有Cu2+与四环素或阿莫西林或红霉素,Cr6+与头孢拉定。环境中重金属离子的共存将改变抗生素污染物的生态危害和环境行为,并最终影响对应的污染防治技术的开发和应用。To study the effect of heavy metal stress on bacterial antibiotic resistance, a Gram-negative bacteria isolated from swine wastewater in Guangzhou was characterized and identified as Pseudomonas alcaligenes LH7. The minimal inhibition concentration （MIC） of heavy metals （Cu2＋, Zn2＋ and Cr6＋） and antibiotic susceptibility test were determined by the methods of Kirby-Bauer disk diffusion and agar dilution. Furthermore, with the joint effect of type and concentration of heavy metals, the inhibition zone diameter of the tested antibiotics （erythromycin, amoxicillin, cephradine and tetracycline） was measured by vernier caliper. The results showed that the MICs of Cu2＋, Zn2＋ and Cr6＋ were 125 mg/L,125 mg/L and 100 mg/L, respectively. Multiple antibiotic resistance was also observed. The Pseudomonas alcaligenes LH7 showed resistance to erythromycin, amoxicillin, cephradine and tetracycline. The interaction of antibiotics and heavy metals significantly affected bacterial resistance （P﹤0.05）. There were three kinds of cross-effects between antibiotics and heavy metals according to the types and concentrations of heavy metals. Firstly, the effect of heavy metals on antibiotic resistance could be co-resistance at low concentrations but synergic germicidal at high concentrations, such as Cr6＋ and erythromycin, Zn2＋ and erythromycin, Cu2＋ and cephradine. Secondly, the effect of heavy metals on antibiotic resistance could be synergic germicidal at low concentrations but co-resistance at high concentrations, such as Cr6＋ and amoxicillin, Zn2＋ and amoxicillin. Finally, the antibiotic resistance was only associated with the types of heavy metal, such as Cu2＋ and tetracycline or amoxicillin or erythromycin, Cr6＋ and cephradine. Co-existence of heavy metals in environment will change the ecological harm and environmental behavior of antibiotics, and affect the development and application of relative pollution prevention techniques, so this is a field worthy of attention.

关 键 词：养猪场废水 PSEUDOMONAS ALCALIGENES LH7 重金属胁迫 抗生素 协同抗性 协同杀菌 

分 类 号：X713[环境科学与工程—环境工程] X172