电针“内关”穴对心肌缺血大鼠ASIC2、ASIC3蛋白及基因表达影响的实验研究  

The Effect of Electro-acupucture on the Neiguan(PC6) Acupoint in the Protein and Genomic Expressions of ASIC2 and ASIC3 in Myocardial Ischemia Rats

作　　者：王树东[1] 董宝强[1] 张立德[1] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110847

出　　处：《中华中医药学刊》2015年第6期1360-1363,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家重点基础研究发展计划(973计划)项目(2012CB518503)

摘　　要：目的:实验通过电针"内关"穴,观察其对心肌缺血大鼠ASIC2、ASIC3蛋白及基因表达的影响。方法:选用健康SPF级雄性SD大鼠50只,体质量(230±50)g,由辽宁长生生物技术有限公司提供,采用随机对照方法分为正常组(A)、模型组(B)、内关组(C)、列缺组(D)、非经非穴组(E),每组10只。四肢皮下多点注射异丙肾上腺素。剂量:85 mg/kg,日1次,连续两次成模。造模成功后,分别进行电针治疗。1周后,各组SD大鼠腹腔注射水合氯醛(1 m L/100 g),活体分离大鼠左心室心肌,置于EP管中,放入-70℃冰箱,Western blot法检测各组ASIC2、ASIC3蛋白表达,Realtime RT-PCR法检测各组ASIC2、ASIC3的基因表达,并应用2^(-△Ctof)得出各组大鼠ASIC2、ASIC3的基因表达多少。腹主动脉抽血,离心后通过ELISA法检测肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)含量。结果:电针"内关"穴可降低心肌缺血大鼠ASIC2、ASIC3蛋白及基因表达,降低肌酸激酶同工酶(CK-MB)、乳酸脱氢酶(LDH)含量,与模型组比较有显著差异(P<0.01)。结论:电针"内关"穴可降低心肌缺血大鼠ASIC2、ASIC3蛋白及基因表达,从而抑制酸敏感离子通道开放,有效改善心肌缺血大鼠心肌细胞的损伤。Objectives： The aim of the study is to explore the effect of electro- acupucture on the Neiguan（ PC6） acupoint in the protein and genomic expressions of ASIC2 and ASIC3 in myocardial ischemia rats. Methods： 50 healthy SPF male SD rats were used with a mass of（ 230 ± 50） g,provided by Liaoning Changsheng Biotechnology Co,Ltd. The rats were randomly split into groups of 10,consisting normal group（ A）,model group（ B）,Neiguan（ PC6） group（ C）,Lieque（ LU7） group（ D）,random acupoint group（ E）. The rats were continuously administered with 85 mg / kg once a day with intravenous isoprenaline（ ISO）. The myocardial ischemia rats were given electro- acupuncture treatment. Each group rats were given chloraldurate（ 1 m L /100 g） intraperitoneal injection. The left ventricle myocardium was extracted and placed in- 70 ℃ before use. Western blot analysis and RT- PCR were performed to check for the protein and genomic expression of ASIC2 and ASIC3. The genomic expression was quantified by 2 ^（- ΔCtof）. Blood samples were drawn from aorta abdominalis,centrifuged and tested for CK- MB,and LDH levels after ELISA. Results： Electro- acupuncture of Neiguan（ PC6） acupoint can decrease the protein and genomic expression of ASIC2 and ASIC3,CK- MB and LDH levels of myocardia ischemic rats. Conclusion： Electro- acupuncture of Neiguan（ PC6） can decrease the protein and genomic expression of ASIC2 and ASIC3,thereby inhibiting the opening of acid- sensing ion channels（ ASICs） and decreasing the damage to the myocardiocytes in myocardial ischemia rats.

关 键 词：内关 ASIC2 ASIC3 蛋白 基因 

分 类 号：R245[医药卫生—针灸推拿学]