天灸防治大鼠肝纤维化的实验研究  

Experimental Study of Vesiculation Moxibustion for Prevention and Treatment of Rat Hepatic Fibrosis

作　　者：刘文吉[1] 李胜杰[1] 罗钦[1] 冀美琦 张伟[1] 解秸萍[1] 

机构地区：[1]北京中医药大学,北京100029

出　　处：《上海针灸杂志》2015年第5期460-463,共4页Shanghai Journal of Acupuncture and Moxibustion

摘　　要：目的观察天灸防治大鼠肝纤维化(HF)的作用。方法将30只大鼠随机分为治疗组、模型组和对照组,每组10只。采用四氯化碳腹腔注射造模的方法建立HF大鼠模型,治疗组从造模后第3星期开始进行天灸治疗,每星期2次,每次6 h,共治疗4星期。观察各组大鼠体重、肝湿重、肝指数,比较血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)及血清和肝脏Ⅲ型前胶原(PCⅢ)、IV型胶原(IV-C)、透明质酸(HA)、层粘连蛋白(LN)的含量。结果模型组大鼠HF程度分级及HF组织胶原分析指标与治疗组和对照组比较,差异均具有统计学意义(P<0.01,P<0.05)。模型组大鼠体重、肝湿重及肝指数与对照组比较,差异均具有统计学意义(P<0.01)。治疗组大鼠肝湿重和肝指数与模型组比较,差异均具有统计学意义(P<0.01)。模型组大鼠血清ALT、AST含量及血清及肝脏PCIII、IV-C、HA、LN含量与对照组比较,差异均具有统计学意义(P<0.01,P<0.05)。治疗组大鼠血清ALT、AST含量及血清及肝脏IV-C、HA、LN含量与模型组比较,差异均具有统计学意义(P<0.01,P<0.05)。治疗组大鼠血清PCⅢ含量与模型组比较,差异具有统计学意义(P<0.01)。结论天灸具有一定的防治大鼠肝纤维化的作用。Objective To investigate the preventive and therapeutic effects of vesiculation moxibustion on rat hepatic fibrosis （HF）. Methods Thirty rats were randomly allocated to treatment, model and control groups, 10 rats each. A rat model of HF was made by intraperitoneal injection of carbon tetrachloride. At three weeks after model making, the treatment group was treated by vesiculation moxibustion 6 hrs twice a week, for a total of 4 weeks. Body weight, liver wet weight, liver index, serum alanine aminotransferase （ALT） and aspartate aminotransferase （AST） contents, and serum and liver type Ⅲ procollagen （PC Ⅲ）, type Ⅳ collagen （Ⅳ-C）, hyaluronic acid （HA） and laminin （LN） contents were measured in every group of rats. Results There were statistically significant differences in HF severity grade and HF tissue collagen analysis indices between the model group of rats and the treatment group or the control group （P〈 0.01,P〈 0.05）. There were statistically significant differences in body weight, liver wet weight and liver index between the model and control groups of rats （P〈0.01）. There were statistically significant differences in liver wet weight and liver index between the treatment and model groups of rats （P〈0.01）. There were statistically significant differences in serum ALT and AST contents, and serum and liver PC III, IV-C, HA and LN contents between the model and control groups of rats （P〈0.01,P〈0.05）. There were statistically significant differences in serum ALT and AST contents, and serum and liver IV-C, HA and LN contents between the treatment and model groups of rats （P〈0.01,P〈0.05）. There was a statistically significant difference in serum PC III content between the treatment and model groups of rats （P〈0.01）. Conclusion Vesiculation moxibustion has some preventive and therapeutic effects on rat hepatic fibrosis.

关 键 词：穴位贴敷法 发泡疗法 肝纤维化 大鼠 

分 类 号：R2-03[医药卫生—中医学]