番茄对南方根结线虫抗性遗传规律的研究  

Inheritance of resistance to Meloidogyne incognita in tomato

作　　者：郑积荣[1] 王慧俐[2] 

机构地区：[1]杭州市农业科学研究院,杭州310024 [2]杭州万向职业技术学院,杭州310023

出　　处：《安徽农业大学学报》2015年第3期463-467,共5页Journal of Anhui Agricultural University

基　　金：杭州市科委项目(20120332H01);浙江省农业新品种选育重大科技专项子课题(2012C12903-1-4);杭州市科研院所专项(20132231E06)共同资助

摘　　要：以南方根结线虫的抗病亲本P2(0897-2-1-2-3-1-2-1)、感病亲本P1(9905-1-2-1-1-1-1-1)及F1(P1×P2),F2(F1自交后代),BC1P1(Fl×P1)和BC1P2(Fl×P2)为试材,以南方根结线虫二龄幼虫为虫源,进行苗期二龄幼虫人工接种鉴定和病土盘栽自然发病鉴定,研究番茄南方根结线虫病的遗传规律。结果表明,人工接种鉴定的植株在接种15 d左右,感病植株开始发病,接种45 d后感病植株已充分发病,表现为植株矮小,叶片变小、变黄,部分植株下部叶片黄枯,有的枯死;病土盘栽自然发病鉴定的植株较苗期接种鉴定的植株迟12 d左右发病,病症相似。卡方检测表明,F1、F2、BC1P1和BC1P2群体中抗病植株和感病植株分别符合1:0、3:1、1:1和1:0基因模型,符合孟德尔遗传规律。认为该试验所采用的抗性材料P2对番茄南方根结线虫的抗性是受一对显性基因控制的,为该材料的利用奠定基础,也为抗南方根结线虫番茄品种的选育提供参考。Tomato（Solanum lycopersicum L.） parental lines, P2（0897-2-1-2-3-1-2-1） resistant to Meloidogyne incognita, and P1（9905-1-2-1-1-1-1-1） susceptible to M. incognita,F2,BC1P1（F1×P1） and BC1P2（Fl×P2） were selected to explore the inheritance of resistance to M. incognita in tomato. Second-stage juveniles（J2s） of M. incognita were directly inoculated to the seedlings mentioned above and added to the soil in cell flats to identify the disease regularity of J2 s in tomato. The results showed that plants susceptible to M. incognita showed the first symptom at the 15 th day after inoculation and exhibited severe symptoms at the 45^th day after inoculation with the symptom of short plants, smaller and yellow leaves, the lower leaves being yellowing and withering, and some plants even being dying. Plants in cell flats containing J2 s showed the first symptom 12 days later than plants with seedling inoculation. The disease symptoms of the two treatments were similar. Resistant plants grew normally. A chi-square（X2） test of the frequency distribution based on the F1, F2, BC1 P and BC1P2 progenies of the two crosses（resistant×susceptible） showed the best-fit for 1（R）：0（S）, 3（R）：1（S）, 1（R）：1（S）,1（R）：0（S） and Mendelian ratio, respectively. The resistant and susceptible plants of individual generations showed a regular distribution. Aptness examination showed that the resistance of parent P2（0897-2-1-2-3-1-2-1） to M. incognita was controlled by one dominant gene. This study would lay a foundation for the utilization of the resistant material and it would also offer reference for breeding tomato resistant to the southern root knot nematodes.

关 键 词：番茄 南方根结线虫 抗性 遗传规律 

分 类 号：S433.1[农业科学—农业昆虫与害虫防治]