姜黄素调控长链非编码RNA AK125910的表达介导肝癌细胞凋亡  

Expression of Long Non-coding RNA AK125910 in Liver Cancer Cell Apoptosis Undergoing Curcumin Regulation

作　　者：过忆[1,2] 周留勇[3] 尤建良[3] 薛博瑜[1] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210023 [2]无锡市中医医院脾胃病科,江苏无锡214071 [3]无锡市中医医院肿瘤科,江苏无锡214071

出　　处：《南京中医药大学学报》2015年第3期254-257,301,共4页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家科技重大专项(2008ZX10005009);无锡市医院管理中心科研项目(YGZXL1306)

摘　　要：目的 检测姜黄素诱导肝癌细胞凋亡中lncRNA表达谱的改变及关键lncRNA的筛选鉴定。方法 使用不同浓度姜黄素刺激肝癌细胞株HepG2不同时间后,提取总RNA进行lncRNA芯片杂交检测lncRNA表达谱的改变,筛选出差异表达lncRNA;并利用MTT法和TUNEL染色观察HepG2细胞的凋亡,Real-time PCR验证差异表达的lncRNA AK125910在其中的作用。结果 与对照组细胞相比,姜黄素处理后的肝癌细胞中有5 432条lncRNA表达出现改变,而其中变化倍数大于3的lncRNA有8条,表达差异化最显著的是lncRNA AK058003,上调7.62倍。在姜黄素诱导肝癌细胞凋亡中,lncRNA AK125910表达上调7.16倍,与芯片杂交结果基本一致。结论 姜黄素诱导肝癌细胞凋亡过程中lncRNA表达谱出现显著变化,其中差异性表达最显著的lncRNA AK125910可能参与了肝癌细胞的凋亡进程。ABSTRACT：OBJECTIVE To detect the apoptosis of liver cancer cell induced by curcumin and related lncRNA expression changes and key lncRNA spectrum screening.METHODS After using different concentrations of curcumin to stimulate HepG2 cells at different times,total RNA was extracted to determine the changes of ncRNA microarray expression profiling ;and cell apoptosis of HepG2 cells was measured by using MTT assay and TUNEL staining.Real-time PCR was performed to examine the differentially expressed lncRNA AK125910.RESULTS Compared with control cells,5432 lncRNA expression in HepG2 cells induced by curcumin were changed,in which eight lncRNAs showed fold change〉 3,and the most significant ex-pression differences is lncRNA AK058003 by up-regulation of 7.62 times.Curcumin induced apoptosis in liver cancer and ln-cRNA AK058003 was upregulated by 7.16-fold,consistent with the results of microarray.CONCLUSION The lncRNA ex-pression profiling in curcumin-induced apoptosis of liver cancer was significant changed.Moreover,most significant differenti-ally expressed lncRNA AK058003 may be involved in apoptosis of liver cancer cells.

关 键 词：长链非编码RNA 姜黄素 AK125910 肝癌细胞 凋亡 

分 类 号：R285.5[医药卫生—中药学]