参希胶囊对内毒素血症小鼠心脏的保护作用  

Protective Effect of Shen Xi Capsule on Endotoxemia in Mice Heart

作　　者：朱明慧[1] 黄礼兵[2] 朱娟[2] 周玉弟[2] 郑曼[2] 

机构地区：[1]南京中医药大学第一临床医学院,江苏南京210023 [2]南京中医药大学附属医院,江苏南京210029

出　　处：《南京中医药大学学报》2015年第3期258-260,共3页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：国家自然科学基金(81202802)

摘　　要：目的探讨参希胶囊对内毒素血症小鼠心脏的保护作用。方法实验小鼠分为4组:对照组、模型组、参希组、参希+模型组,采用腹腔注射内毒素(LPS)制备小鼠内毒素血症模型,对照组和参希组不予腹腔注射LPS。参希组及参希+模型组于造模前28d给予参希胶囊(0.75g/kg)灌胃,余组给予等体积的生理盐水灌胃。分别于造模后6h行超声心动图测定心功能,检测心肌TNF-、IL-1和MDA的含量,测定SOD的活性。结果与对照组比较,模型组小鼠的左室射血分数(EF)和短轴缩短率(FS)明显降低(P<0.05),参希预处理显著提高内毒素血症小鼠的EF和FS值(P<0.05);与对照组比较,模型组小鼠心肌TNF-、IL-1和MDA含量升高,SOD的活性降低(P<0.05),参希预处理显著减少内毒素血症小鼠心肌TNF-、IL-1和MDA含量,提高SOD的活性(P<0.05)。结论参希胶囊对内毒素血症小鼠心脏具有保护功能,其可能与降低心脏炎症因子水平和氧化应激反应有关。ABSTRACT：OBJECTIVE To explore the protective effects of shenxi on heart in endotoxemic mice.METHODS The experi-mental mice were equally and randomly divided into control group,LPS group,shenxi group,shenxi＋LPS group.The endo-toxemia model was established by intraperitoneally injecting LPS.Mice in groups shenxi and shenxi＋LPS were intragastrically administered with shenxi solution（0.75 g/kg）,while the same dose of normal saline were intragastrically administrated in con-trol group and LPS group.The heart function was detected with echocardiography,and TNF-α,IL-1β,MDA,SOD in myocar-dial tissue was estimated 6 hours after LPS administration.RESULTS Compared with control group,LPS treatment could re-sult in decreased myocardial activities of SOD,and elevated levels of TNF-α,IL-1β,MDA（P 〈0.05）;shenxi treatment could improve the above aberrant indices.Compared with control group,LPS treatment could result in reduced cardiac function, while shenxi treatment could improve it.CONCLUSION Shenxi shows a good cardio-protective effect on the cardiac injury in endotoxemic mice,which may be reached by suppressing inflammatory infiltration and oxidative stress reaction.

关 键 词：内毒素血症 心肌损伤 炎性因子 氧化应激 

分 类 号：R285.5[医药卫生—中药学]