丹参酮Ⅰ脂质体的制备及表征  

Preparation and characterization of TanshinoneⅠ-loaded Liposomes

作　　者：郭丽丽[1,2] 范丽丽[1,2] 王爱潮 庞晓晨[1,2] 罗配 杨红云[1,3] 王少峡[1,3] 刘志东[1,2] 

机构地区：[1]天津市现代中药重点实验室天津中医药大学,天津300193 [2]天津中医药大学现代中药发现与制剂技术教育部工程研究中心,天津300193 [3]天津中医药大学中医药研究院天津市现代中药重点实验室--省部共建国家重点实验室天津市中药药理学重点实验室,天津300193

出　　处：《天津中医药》2015年第5期308-311,共4页Tianjin Journal of Traditional Chinese Medicine

基　　金：教育部新世纪优秀人才支持计划(NCET-12-1068)

摘　　要：[目的]制备丹参酮Ⅰ脂质体,并对其包封率、粒径、电位等理化性质进行考察。[方法]采用薄膜分散法丹参酮Ⅰ脂质体,用琼脂糖凝胶柱色谱法和紫外分光光度法测定包封率,用差示扫描量热法检测丹参酮Ⅰ脂质体各组成物质的相变过程。[结果]丹参酮Ⅰ在1.004~6.024μg/m L范围内线性关系良好（r=0.999 9）,琼脂糖凝胶柱色谱法能有效分离脂质体和游离药物,加样回收率为（98.23±0.02）%,平均包封率为（92.56±0.39）%,粒径为（90.64±1.21）nm,电位为（-35.37±0.84）m V。[结论]薄膜分散法可用于制备丹参酮Ⅰ脂质体,制备的丹参酮Ⅰ脂质体的包封率高,粒径均一,电位稳定,药物含量和包封率测定方法准确可靠,专属性强。[Objective] To prepare TanshinoneⅠ-loaded Liposomes（TSⅠ-LP） and investigate its entrapment efficiency（EE）, particle size, Zeta potential and other physicochemical properties. [Methods] TS Ⅰ-LP were prepared by film dispersion method, and using Sepharose gel and UV spectrophotometry to determine the entrapment efficiency of TS Ⅰ-LP. Differential scanning calorimetry（DSC）thermograms of the liposomal samples were recorded respectively. [Results] A good linear relationship was showed of TSⅠ in the range of1.004 ~6.024 μg/m L（r =0.999 9）. Sepharose gel was well in separating the liposomes from free TS Ⅰ. The average recovery rate was（98.23±0.02）%. The EE of TSⅠ-LP was（92.56±0.39）%. The particle size was（90.64±1.21） nm and the Zeta potential was（-35.37±0.84） m V. [Conclusion] The film dispersion method can be used to prepare TSⅠ-LP with a high EE, a narrow size distribution and a stationary potential. The method which determine the content of TSⅠ-LP is reliable and sensitive.

关 键 词：丹参酮Ⅰ 脂质体 包封率 

分 类 号：R284.2[医药卫生—中药学]