多孔菌漆酶在AOT/异辛烷反胶团中的特性表征  

Characterization of Polyporous Laccase in AOT / Isooctane Reversed Micelles

作　　者：张俊 潘易 林宝达 周春何 董海洁 

机构地区：[1]丽水市环境监测中心站,浙江丽水323000 [2]丽水市固体废物管理中心,浙江丽水323000

出　　处：《安徽农业科学》2015年第18期5-7,共3页Journal of Anhui Agricultural Sciences

摘　　要：[目的]对多孔菌漆酶包埋于AOT/异辛烷反胶团中的特性进行研究。[方法]在漆酶反胶团中加入醋酸盐缓冲液和邻联甲苯胺,放入25℃恒温箱中培养25 min后离心,取下清液,测光密度值。[结果]在漆酶反胶团体系中加入适量吐温-80后,漆酶反胶团酶活力有所提高。经反胶团包埋后,酶的热稳定性和酸碱稳定性较水相酶有较大提高,在40℃下保温30 d酶活力保留81.3%,在p H 4.5下保存30 d酶活力保留89.6%。[结论]在该反胶团体系中,酶的稳定性有较大的提高。[ Objective ] The characteristics of polyporous laccase entrapped in AOT/isooclane reversed micelles were studied. [ Method ] Ace- late buffer and o-tolidine into AOT/isooetane reversed micelles was added, then was put in an incubator chamber at 25 degrees for 25 minutes. Bottom clear liquid was collected to measure optical density value after centrlfugating. [ Result ] Its activity was enhanced after joining optimal quantity of Tween-80 and its stability to variation of temperature and pH was improved to a great extent. 81.3% of its activity still remained after storage at 40 ℃ for 30 days,and 89.6% of its activity remained after storage at pH 4.5 for 30 days. [ Conclusion] The stability of pulyporous lac- case was enhanced greatly in the reversed mieelles.

关 键 词：多孔菌漆酶 AOT 异辛烷 反胶团 特性表征 

分 类 号：S-03[农业科学]