利用宏基因组技术发掘新木聚糖酶基因  

Developing Novel Xylanase Genes with Metagenomic Technology

作　　者：罗阳[1] 何波[1] 王佳堃[1] 刘建新[1] 

机构地区：[1]浙江大学奶业科学研究所,动物分子营养学教育部重点实验室,浙江杭州310058

出　　处：《中国畜牧杂志》2015年第11期86-91,共6页Chinese Journal of Animal Science

基　　金：科技部国际合作项目(2010DFA31040)

摘　　要：木聚糖酶将日粮中的木聚糖降解成低聚糖。低聚糖及其厌氧发酵产物、短链脂肪酸可促进消化道发育,维持消化道微生物稳态。发掘新高效的木聚糖酶一直是酶工程研究的热点。宏基因组技术为此研究提供了保障。BAC和Fosmid宏基因组文库承载的信息量大,但阳性克隆率低,加大了分析工作量。木聚糖酶功能域由保守区和可变区嵌合构成,这为利用功能域基因研究木聚糖酶基因的多样性及其与微生物菌群的关系提供了条件。为此,本文在总结纯培养技术优缺点的基础上,重点归纳了BAC和Fosmid宏基因组文库及木聚糖酶功能域基因文库在发掘新木聚糖酶基因的应用。The oligosaccharides producted by xylanase from dietary hemicelluloses, and their microbial fermentation product, short-chain fatty acids, can promote growth of gut and maintain the balance of micro-environment. Therefore, development of new and efficient xylanase has been a hot research area in enzyme engineering. Metagenomic technology is a good tool for developing novel xylanase genes. There ismuchbiological information in the metagenomic library, such as BAC and fosmid, but thelow rate of positive cloningleads to a heavy analysis workload. The specific chimeric structure of full-lengthxylanase geneis consisted by conservative and variable domains. This provides opportunities to study diversity of xylanase genes and its relationship with microbiota. In this paper, we summarized the advantages and disadvantages of culture-dependent technology, and the application of BAC and fosmid metagenomic libraries and xylanase domains library in developing new xylanase gene.

关 键 词：木聚糖酶基因 宏基因组技术 纯培养技术 

分 类 号：S816.7[农业科学—饲料科学]