基于三种叶绿体基因序列的证据分析阴石粉背蕨和金粉背蕨的关系  

The Relationship between Aleuritopteris humatifolia and A. chrysophylla Based on Three Chloroplast DNA Sequences Analyses

作　　者：王弯[1] 杨文利[2] 窦平[1] 赵冉冉[1] 张钢民[1] 

机构地区：[1]北京林业大学自然保护区学院,北京100083 [2]河北农业大学园林与旅游学院,河北保定071000

出　　处：《植物分类与资源学报》2015年第3期301-308,共8页Plant Diversity

基　　金：国家自然科学基金(31270253);国家基金委重大国际合作项目(31110103911)

摘　　要：选择阴石粉背蕨(Aleuritopteris humatifolia)和金粉背蕨(A.chrysophylla)及其相关类群共27种植物,提取基因组DNA,通过聚合酶链式反应(PCR)扩增其叶绿体rbc L,trn L-F和mat K序列,所得序列利用Clustal X软件进行对位排列,用MEGA6.0软件计算种内及种间遗传距离,并采用最大简约法(MP)构建系统发育树。结果显示:阴石粉背蕨1个样品和金粉背蕨2个样品的rbc L,trn L-F和mat K序列间差异很小,最大遗传距离均小于其与其它近缘种的种间最小遗传距离,结合两个种叶片形态、孢子、囊群盖和根状茎鳞片等形态学特征的对比研究,作者认为阴石粉背蕨应该作为金粉背蕨的异名处理。Total DNA from 27 species including Aleuritopteris humatifolia and A. chrysophylla and related taxa was extracted in this research, and the rbcL, trnL-F and matK sequences were amplified and sequenced. The sequences were aligned by ClustalX. The phylogenetic trees were constructed by Maximum parsimony methods and the intraspecific and interspecific genetic distance were computed in MEGA 6. 0. The results showed as follows： no significant difference among rbcL, trnL-F and matK sequences of one sample of A. humatifolia and two samples of A. chrysophylla, and the maximum genetic distance between A. humatifolia and A. chrysophyUa were less than the minimum genetic distance between A. humatifolia and other closely related species. Therefore, our study support the taxonomic treatment of reducing A. humatifolia to synonym of A. chrysophylla based on our phylogenetic analysis combining the evidences from their morphological characters of blades, spores, indusia, and rhizome scales.

关 键 词：阴石粉背蕨 金粉背蕨 碎米蕨类 RBCL trnL—F MATK 

分 类 号：Q949[生物学—植物学]