不同添加剂对BSA-PLGA缓释微球特性的影响  

Effects of different additives on the characteristics of BSA-PLGA sustainedrelease microspheres

作　　者：黄婉丹[1] 洪乐鹏[1] 龙大宏[1] 

机构地区：[1]广州医科大学人体解剖学教研室,广东广州510182

出　　处：《广州医科大学学报》2014年第5期5-8,共4页Academic Journal of Guangzhou Medical University

基　　金：广州医科大学基金项目(L95114);广东省医学科研基金项目(A2013239)

摘　　要：目的:探讨不同添加剂对聚乳酸-聚乙二醇酸(PLGA)包裹牛血清白蛋白(BSA)制备的BSA-PLGA微球特性的影响。方法:采用复乳-溶剂挥发法制备BSA-PLGA微球,显微测量微球粒径,以微量BCA法测定微球的蛋白含量并计算包封率,进行体外释放,测定微球的累积释放量。探索添加剂PEG6000、Tween-20、Trehalose对微球粒径、包封率、突释量和累积释放量的影响。结果:加入添加剂制备的微球,粒径增大,突释降低,载药量、包封率、累积释放量均有所提高,且呈浓度依赖性(P<0.05)。结论:通过加入适当种类和浓度的添加剂,可以得到较高包封率和累积释放量、较小突释、适当载药量和粒径的BSA-PLGA微球。Objective：To investigate the effects of different additives on the characteristics of BSA-PLGA sustained-release microspheres prepared by polylactic acid-glycolic acid （ PLGA） encapsulation of bovine serum albumin （ BSA ） . Methods：BSA-PLGA microspheres were prepared by double emulsion/solvent evaporation method.The diameter of microspheres was measured by microscope. The protein content of microspheres was determined by micro BCA assay,and the encapsulation efficiency was calculated. Microspheres were released in vitro and the total release amount was detected.The effects of additives PEG6000,Tween-20 and Trehalose on the diameter,encapsulation efficiency,burst release amount and total release amount of microspheres were evaluated. Results：At the presence of additives, the diameter, drug loading capacity, encapsulation efficiency and total release amount of the prepared microspheres increased significantly while the burst release decreased; all these changes were dose-dependent （P〈0.05）.Conclusion：BSA-PLGA microspheres with relatively high encapsulation efficiency and total release amount,low burst release,proper drug loading and particle diameter could be produced by adding suitable types and concentrations of additives.

关 键 词：聚乳酸-聚乙二醇酸 牛血清白蛋白 微球 

分 类 号：R944[医药卫生—药剂学]