尼古丁对大鼠牙槽骨内ALP和DMP1 C末端表达的影响  

The effects of nicotine on the expression of ALP and the DMP1 C terminal in rat alveolar bone

作　　者：邱萌[1] 马肃[1] 侯琳[1] 张宇[1] 陈力[2] 秦春林[2] 

机构地区：[1]哈尔滨医科大学口腔医院牙周科,黑龙江哈尔滨150001 [2]哈尔滨医科大学附属第一医院黑龙江学者实验室,黑龙江哈尔滨150001

出　　处：《临床口腔医学杂志》2015年第5期263-265,共3页Journal of Clinical Stomatology

基　　金：黑龙江省科技厅自然科学基金资助(D2004-43);黑龙江省科技厅科研基金资助(2010-007)

摘　　要：目的:观察尼古丁对大鼠牙槽骨内碱性磷酸酶(ALP)和牙本质基质蛋白1(DMP1)C末端表达的影响。方法:将20只5周龄健康雄性Wistar大鼠随机等分为实验组和对照组,每组再随机等分为30天和60天两个亚组,实验组每天腹腔注射尼古丁0.73 mg·kg-1。分别使用钙钴法和免疫组化法检测ALP和DMP1 C末端在大鼠牙槽骨内的表达。结果:对照组:ALP和DMP1 C末端都为深棕色线形表达于牙槽骨的钙化前缘,形成明显的矿化条带;ALP和DMP1 C末端分别为棕色沉淀和棕黄色颗粒状密布于骨细胞周围。实验组:ALP和DMP1 C末端表达部位同对照组,表达强度减弱;相对于30天组,60天组变化更明显。结论:尼古丁可能通过抑制大鼠牙槽骨内ALP和DMP1 C末端的表达,抑制大鼠牙槽骨的矿化。Objective：To observe the effects of nicotine on the expression of Alkaline Phosphatase（ALP）and the dentin matrix protein（DMP）1 C terminal in rat alveolar bone. Method：Twenty health male Wistar rats of five weeks of age were randomly divided into experimental group and control group,each group was randomly divided into two subgroups：30 and 60 days,the experimental group was to nicotine 0.73 mg·kg^-1·d^-1 by intraperitoneal injection. The expression of ALP and the DMP1 C terminal in the alveolar bone was respectively detected by calcium cobalt method and immun-ohistochemistry. Result：Control group：ALP and DMP1 C terminal linear expressed in the calcification front of the alveolar boneand it is dark brown,apparent mineralization strap was formed;ALP brown precipitation and DMP1 C term-inal brown yellow granular dense clouded around the bone cells;Experimental group：ALP and DMP1 C terminal parts of the same expression in the control group,decreased intensity of expression,the change of 60 days is more obvious than 30 days. Conclusion：Nicotine may inhibit mineralization of rat alveolar bone by declining the expression of ALP and DMP1 C terminal in rat alveolar bone.

关 键 词：尼古丁 牙槽骨 碱性磷酸酶 牙本质基质蛋白1 

分 类 号：Q786[生物学—分子生物学]