控制性灌注血必净注射液对兔后肢缺血再灌注损伤的保护作用  

Protective Effect of Controlled Reperfusion with Xuebijing Injection on Ischemia-Reperfusion Injury of Rabbit Hind Limb

作　　者：王刚[1] 钟红珊[1] 

机构地区：[1]中国医科大学附属第一医院介入科,辽宁沈阳110001

出　　处：《中国普外基础与临床杂志》2015年第5期560-564,共5页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的探讨控制性灌注血必净注射液对后肢缺血再灌注损伤的保护作用,为临床通过灌注血必净防治肢体缺血后再灌注损伤提供理论依据。方法制备兔后肢缺血再灌注模型(缺血2 h,再灌注4 h),将30只健康大耳白兔应用随机数字表完全随机化分为3组:1血必净灌注组(n=10),再灌注前给予血必净;2盐水对照组(n=10),手术方式同1组,再灌注前给予生理盐水灌注;3假手术组(n=10):手术方式同1组,不予缺血及再灌注。检测各组血清中超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量的变化以及后肢CT灌注参数——血流量比值(r BF)和血容量比值(r BV)的变化。结果 1假手术组术前及术后血清中SOD活性、MDA含量未见明显变化(P>0.05);盐水对照组再灌注后血清中SOD活性较术前略升高(P>0.05),MDA含量较术前明显升高(P<0.01);血必净灌注组再灌注后血清中SOD活性较术前明显升高(P<0.01),MDA含量较术前明显降低(P<0.01)。与假手术组比较,血必净灌注组及盐水对照组再灌注后血清中SOD活性明显提高(P<0.01),MDA含量血必净灌注组明显降低(P<0.01),盐水对照组明显升高(P<0.01);与盐水对照组比较,血必净灌注组灌注后血清中SOD活性明显提高(P<0.01),MDA含量明显降低(P<0.01)。2与假手术组比较,盐水对照组及血必净灌注组的r BF及r BV均明显下降(P<0.05);与盐水对照组比较,血必净灌注组r BF及r BV均明显升高(P<0.05)。结论控制性灌注血必净注射液可提高兔血清中SOD活性,降低MDA含量,对兔后肢缺血再灌注损伤有保护作用,并且能有效改善后肢灌注量。Objective To discuss the protection effect of controlled reperfusion with Xuebijing injection on ischemia-reperfusion injury of rabbit hind limb, and provide a theoretical basis for prevention and treatment of limb ischemia reperfusion injury in clinical. Methods The big ear rabbit model of hind limb ischemia-reperfusion injury （ischemia 2 h, reperfusion 4 h） was made. Thirty healthy big ear rabbits were randomly divided into three groups： Xuebijing perfusion group （n=10）： Xuebijing injection was given before reperfusion; Saline control group （n=10）： surgical procedure with Xuebijing perfusion group, saline infusion was given before reperfusion; sham operation group （n=10）： surgical procedure with Xuebijing perfusion group, ischemia and reperfusion was not performed. The activity of serum superoxide dismutase （SOD） and content of malondialdehyde （MDA） were detected. The ratio of blood flow （rBF） and ratio of blood volume （rBV） were tested. Results ① Compared with the levels before operation, the activity of SOD and content of MDA had no significant differences after operation in the sham operation group （P〉0.05）, the activity of SOD was obviously increased and the content of MDA was obviously decreased after reperfusion in the Xuebijing perfusion group （P〈0.01）. Compared with the saline control group, the activity of SOD was obviously increased and the content of MDA was obviously decreased in the Xuebijing perfusion group. ② Compared with the sham operation group, the rBF and rBV were obviously decreased in the Xuebijing perfusion group and the saline control group （P〈0.05）; Compared with the saline control group, the rBF and rBV were obviously increased in the Xuebijing perfusion group （P〈0.05）. Conclusion Controlled reperfusion with Xuebijing injection could increase SOD activity in serum, reduce MDA content, it has a protective effect on hind limb ischemia-reperfusion injury in rabbits, and could effectively improve perfusion of hind limb.

关 键 词：缺血再灌注 控制性再灌注 后肢 血必净注射液 

分 类 号：R543[医药卫生—心血管疾病]