十二指肠空肠旁路术及迷走神经肝支对非肥胖2型糖尿病大鼠糖代谢的影响  

Impact of Duodenal Jejunal Bypass and Hepatic Branch of Vagus on Glucose Metabolism in Non-Obese Rat with Type 2 Diabetes Mellitus

作　　者：万鹏[1] 王瑜[1] 

机构地区：[1]南京军区福州总医院普通外科,福建福州350025

出　　处：《中国普外基础与临床杂志》2015年第5期565-570,共6页Chinese Journal of Bases and Clinics In General Surgery

基　　金：南京军区医学科技创新重点项目(编号:09Z031);福建省科技合作重点项目(编号:2060801)~~

摘　　要：目的观察十二指肠空肠旁路术(DJB)及迷走神经肝支对非肥胖2型糖尿病大鼠糖代谢及空腹血清胰高血糖素样肽-1(GLP-1)、酪酪肽(PYY)的影响及相互作用。方法将40只非肥胖2型糖尿病的Wistar大鼠(GK大鼠)随机分成DJB组、DJB+迷走神经肝支离断组(简称"DJB+HBVR组")、假手术组、假手术+迷走神经肝支离断组(简称"假手术+HBVR组")。检测各组术前及术后体质量及空腹血糖、空腹血清胰岛素水平、血清GLP-1及PYY含量变化。结果 DJB组术后各时相与术前相比大鼠体质量和空腹血糖水平均明显下降(P<0.05),空腹血清胰岛素水平、空腹血清GLP-1及PYY含量显著升高(P<0.05)。与DJB+HBVR组比较,发现从第6周开始,保留迷走神经肝支可以更持久地维持术后低体质量(P<0.05),增加空腹血清GLP-1及PYY含量(P<0.05)。结论 DJB改善GK大鼠糖代谢效果显著,迷走神经肝支在DJB改善糖代谢这一过程中也起到一定作用。Objective To observe the influence and interaction of duodenal jejunal bypass （DJB） and hepatic branch of vagus on glucose metabolism, and fasting serum glucagon like peptide -1 （GLP-1）, peptide YY （PYY） in non-obese rat with type 2 diabetes mellitus （T2DM）. Methods Forty non-obese Wistar rats （GK） with T2DM were randomly divided into four groups： sham operation group （SO group）, sham operation plus hepatic branch of vagus resection （HBVR） group （SO＋HBVR group）, DJB group, and DJB＋ HBVR group. The changes of preoperative and postoperative body weight, fasting blood glucose level, fasting serum insulin level, fasting serum GLP- 1 and PYY contents among four groups were observed. Results In the D]B group, the postoperative body weight and fasting blood glucose level were decreased significantly （P〈0.05） and the fasting insulin level, fasting serum GLP-1 and PYY contents were increased significantly （P〈0.05） as compared with the preoperative corresponding values in the same group, and it was found that the hepatic branch of vagus could more lastingly maintain postoperative lower body weight （P〈0.05）, improve the level of insulin （P〈0.05）, increase the fasting serum GLP-1 and PYY contents （P〈0.05） as compared with the DJB＋HBVR group. Conclusion DJB could improve glucose metabolism effect of GK rats, the hepatic branch of vagus might play a role in it, too.

关 键 词：2型糖尿病 十二指肠空肠旁路术 迷走神经肝支 胰高血糖素样肽-1 酪酪肽 

分 类 号：R656.6[医药卫生—外科学]