正常新生儿血、尿肠型脂肪酸结合蛋白浓度的探讨  

The Blood and Urine Concentration of Intestinal Fatty Acid Binding Protein in Normal Newborns

作　　者：田云粉[1] 李利[1] 米弘瑛[1] 皇甫春荣[1] 何山[1] 许小艳[1] 曹永久 

机构地区：[1]云南省第一人民医院儿科,云南昆明650032 [2]云南省第一人民医院遗传诊断中心,云南昆明650032

出　　处：《昆明医科大学学报》2015年第5期52-54,95,共4页Journal of Kunming Medical University

基　　金：云南省应用基础研究计划面上项目(2012FB204)

摘　　要：目的探讨正常新生儿血、尿肠型脂肪酸结合蛋白(intestinal fatty acid binding protin,I-FABP)跟性别、胎龄、出生史等的关系,了解血、尿中的相关性.方法收集云南省第一人民医院2012年12月至2013年4月NICU收治的没有感染、窒息病史的早产儿100例及同期出生的正常足月儿100例为实验对象,出生后24 h内采外周静脉血2 m L及用无菌管收集尿液2 m L,离心,收上清液,-60℃冰箱保存,统一检测.用ELASA方法测定血及尿液中IFABP的浓度.结果新生儿血、尿中I-FABP在性别、胎龄、出生史进行比较差异无统计学意义(P>0.05).血、尿中I-FABP有相关性,Spearman相关系数为0.489(P<0.05).结论血、尿中I-FABP浓度与新生儿胎龄、性别、出生史、单胎、多胎等因素无关.血尿中IFABP浓度有相关性,血标本因易导致医源性贫血及依从性差,而尿液I-FABP的检测采集方便、成本低、病人痛苦少等特点,临床上可以用尿液替代血液进行检测.Objective To investigate the correlation between the blood and urine concentration of intestinal fatty acid binding protein （Intestinal fatty acid binding protin, I-FABP） with gender, gestational age, birth history in normal newborns, and to understand the blood and urine I-FABP correlation. Methods In our hospital from December 2012 to April 2013, 100 cases of newborns without infection and suffocation and 100 cases of full-term newborns born in the same period in our department NICU were enrolled in this study. The 2mL peripheral vein blood and 2 mL urine samples within 24 hours after birth were collected with a sterile tube and centrifuged for 20 minutes or so （ 2 000-3 000 r/min ） , the supernatants were collected, and stored in -60 degrees refrigerator. Blood and urine IFABP concentration was determined by ELASA methods. Results There were no statistically significant differences in the neonatal blood, urine concentrations of I-FABP between different gender, gestational age, and birth history （P〉0.05） . Blood and urine concentrations of I-FABP were correlated, the Spearman correlation coefficient was 0.489 （P〈0.05） Conclusions The blood, urine concentrations of I-FABP in newborns have no correlation with gender, birth history, single births, multiple births and other factors. The blood and urine concentrations of I-FABP are correlated. Because blood samples easily lead to iatrogenic anemia and poor compliance, and urine samples are easily collected with low cost and less patient pain, so urine samples can be used to substitute blood samples for clinical detection.

关 键 词：肠型脂肪酸结合蛋白 新生儿 正常 

分 类 号：R722[医药卫生—儿科]