远端缺血再灌注预处理对心肌细胞膜ATP敏感性钾离子通道表达的影响  

Effect of remote ischemic preconditioning on the expression of myocardial ATP-sensitive potassium channel

作　　者：徐林[1] 张登文[1] 杨仕杰[1] 田学愎[1] 王煜[1] 韩爱迪[1] 张传汉[1] 田玉科[1] 王学仁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科

出　　处：《中国临床药理学与治疗学》2015年第4期366-369,共4页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金(81371251);国家临床重点专科建设项目资助

摘　　要：目的：观察远端肢体缺血再灌注预处理后心肌细胞膜KATP通道在不同时点的表达变化。方法： 夹闭大鼠双侧股动脉5 min 后开放5 min，如此重复3次完成远端肢体缺血再灌注预处理，分别于处理后1、4、24和48 h处死大鼠，取其左心室肌组织，检测其心肌细胞膜KATP通道各亚基表达变化。结果： 远端肢体缺血再灌注预处理后，心肌细胞膜KATP通道的表达量增加，在4～24 h内增加最明显。 结论：远端肢体缺血再灌注预处理可上调心肌细胞膜KATP通道的表达，这种表达的改变可能参与了远端肢体缺血再灌注预处理对心脏的保护作用机制。AIM： To investigate the expression of sarcolemmal ATP-sensitive potassium channel in cardiomyocytes after remote ischemic preconditioning （RIPC）. METHODS ： Rat models of remote ische- mic preconditioning were established by clipping bi- lateral femoral artery 5 minutes and opening 5 mi- nutes for 3 cycles. Then, the expression of KATP channel in cardiomyocytes was evaluated in control group and groups at lh, 4h, 24h and 48h postopera- tively. RESULTS： The relative protein expression level of sarcolemmal ATP-sensitive potassium chan-nel in cardiomyocytes was increased after RIPC. CONCLUSION： The expression of sarcolemmal ATP-sensitive potassium channel was increased after RIPC, the change of expression in ATP-sensitive po- tassium channel may play a role in the cardiac pro- tection of RIPC against ischemia.

关 键 词：KATP通道 心肌保护 缺血再灌注损伤 

分 类 号：R965.2[医药卫生—药理学]