左旋氨氯地平对预收缩的大鼠离体基底动脉的舒血管活性作用及其机制研究  

Vasodilative activity of levamlodipine in isolated rat basilar artery and its underlying mechanisms

作　　者：潘阳新 史圆圆[2] 陈健[1] 谭盛[1] 

机构地区：[1]南方医科大学珠江医院神经内科,广州510282 [2]南方医科大学第二临床医学院,广州510282

出　　处：《中华神经医学杂志》2015年第5期438-442,共5页Chinese Journal of Neuromedicine

基　　金：广东省临床用药研究基金，广东省大学生创新创业训练计划项目

摘　　要：目的 探讨左旋氨氯地平对缺氧状态下5-羟色胺（5-HT）预收缩的大鼠离体基底动脉的舒血管活性作用及其作用机制.方法 选择SD大鼠离体基底动脉为实验对象,分为去内皮血管组和内皮完整血管组,去内皮血管组采用低离子强度营养液灌注法将取材血管去除内皮.实验期间持续向浴槽内通入混合气体,依实验目的不同在常氧时通入95％O2＋5％CO2混合气体,在缺氧时通入95％N2＋5％CO2混合气体.2组血管均进行血管稳定性检测,检测合格后采用5×10^-7 mol/L5-HT预收缩血管,（1）在缺氧状态下分别用不同浓度（10^-8～10^-3 mol/L）左旋氨氯地平孵育15 min,采用血管张力测定系统检测各个时间点的血管直径;（2）在常氧状态和缺氧状态分别加入10^-4 mol/L左旋氨氯地平孵育15 min和10^-4 mol/L N-硝基-L-精氨酸甲酯（L-NAME）孵育30 min,采用血管张力测定系统检测各个时间点的血管直径.结果 （1）不同浓度（10^-8～10^-3 mol/L）左旋氨氯地平对5-HT预收缩的内皮完整血管组的抑制百分率分别为0.0436％±0.0116％、0.0530％ ±0.0134％、0.0696％ ±0.0103％、0.1265％±0.0284％、0.2362％ ±0.0275％、0.4084％ ±0.0260％、0.5185％ ±0.0238％、0.5358％±0.0160％,对5-HT预收缩的去内皮血管组的抑制百分率分别为0.0607％±0.0084％、0.0852％±0.0138％、0.1676％±0.0247％、0.3285％±0.0250％、0.4161％±0.284％、0.4219％±0.260％,且左旋氨氯地平对缺氧条件下5-HT预收缩的内皮完整血管的抑制作用显著大于去内皮血管,差异均有统计学意义（P＜0.05）.（2）在常氧状态下,10^-4 mol/L左旋氨氯地平对5-HT预收缩血管的抑制率在L-NAME孵育前后分别为0.9626％±0.0224％、0.7358％±0.0149％;在缺氧状态下,10^-4 mol/L左旋氨氯地平对5-HT预收缩血管的抑制率在L-NAME孵育前后分别为0.9485％±0.0129％、0.8979％±0.0150％,比较差异均有统计学意义（P＜0.05）.结论 左Objective To explore the effect of levamlodipine on contraction of isolated rat basilar arteries induced by 5-hydroxytryptamine （5-HT） in hypoxic condition and its mechanism.Methods Basilar arteries in SD rats were applied in this experiment,and divided into vascular endothelial group and endothelium intact group,and the arteries in the vascular endothelial group were perfused low ionic strength solution to remove the vascular endothelium.Mixed gases were passed continuously into the bath liquid during the experiment;according to the different objectives,95% O2＋5% CO2 mixed gas was passed at normoxia condition,while 95% N2＋5% CO2 gas mixture at hypoxia.Blood vessels from the two groups were carried out vascular endothelial integrity and stability test.After passing the test,5× 10^-7 mol/L 5-HT was used to pre-contract blood vessel;（1） vessels were respectively incubated with different concentrations （10^-8-10^-3 mol/L） of levamlodipine for 15 min,and the diameter changes of vessels were determined at each time point using vascular tension system;（2） 10^-4 mol/L L-NAME solution （nitric oxide synthase inhibitor） for 30 min at hypoxia and 10^-4 mol/L of amlodipine for 15 min at normoxia condition were incubated into the vessels,respectively,and the diameter changes of rat basilar artery precontracted by 5-HT were also recorded.Results （1）The inhibitory percentage of levamlodipine at different concentration （10^-8-10^-3 mol/L） in the endothelium intact group was 0.0436%± 0.0116%,0.0530%±0.0134%,0.0696%±0.0103%,0.1265%±0.0284%,0.2362%±0.0275%,0.4084%± 0.0260%,0.5185%±0.0238% and 0.5358%±0.0160%,respectively,and that in the vascular endothelial group was 0.0607%±0.0084%,0.0852%±0.0138%,0.1676%±0.0247%,0.3285%±0.0250%,0.4161%± 0.284% and 0.4219%±0.260%;the inhibiting effect in the endothelium intact group was more obvious than that in the vascular endothelial group at hypoxic condition with significant difference （P〈0.05）.（2） At the normal condition,the inhibitory

关 键 词：左旋氨氯地平 缺氧 基底动脉 血管舒张 

分 类 号：R965[医药卫生—药理学]