光滑鳖甲肽聚糖识别蛋白的生物信息学分析  

Bioinformatics analysis of peptidoglycan recognition protein from Anatolica polita

作　　者：徐鑫[1] 王鹏[1] 刘忠渊[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,新疆乌鲁木齐830046

出　　处：《生物技术》2015年第2期133-137,164,共6页Biotechnology

基　　金：国家自然科学基金项目("新疆荒漠甲虫光滑鳖甲免疫防御机制的初步研究";No.31460578)资助

摘　　要：[目的]预测光滑鳖甲肽聚糖识别蛋白的结构与功能。[方法]采用生物信息学方法对该基因及其编码蛋白的基本理化性质、疏水性、信号肽、二级结构和亚细胞定位等方面进行预测和分析,同时构建其编码产物的系统进化树。[结果]此蛋白的理论分子量为21.882 k D,包含一个由20个氨基酸组成的信号肽,属亲水蛋白,分泌到胞外发挥作用,可能具有信号转导和响应胁迫与免疫反应的功能,与赤拟谷盗的同源性最高,具有能够与肽聚糖结合的保守的PGRP结构域和一个Ⅱ型酰胺酶结构域。[结论]光滑鳖甲肽聚糖识别蛋白Ap PGRP-FD1基因克隆成功,生物信息学分析及结合蛋白质结构与功能预测可为深入研究ApPGRP-FD1提供理论指导。[ Objective] To abtain more information about ApPGRP- FD1 protein from Arratolica polita. [ Methods] Bioinformatics ap- proach were applied to analyze ApPGRP- FD1 protein general physical and chemical properties, hydrophobicity, signal peptide, second- ary structure and localization sites in cells, multiple sequence alignment with its homologous sequences. [ Results ] It was found that the ApPGRP- FD1 protein contains a complete ORF（591bp）encoding 196 amino acids with a predicted molecular weight about 21. 882 kD and pI 6.64. The protein encoded by PGRP gene has a signal peptide consists of 20 amino acids, and it is a hydrophilic protein which is- mainly secreted to extracellular space, the most likely function of this protein was signal transducer, stress response and immune response, has the highest homology with Tribolium castaneum. [Conclusion]The gene ofAnatolicapolita ApPGRP-FD1 had been cloned success- fully. The experimental results of protein structure and function prediction by bioinformatics analysis provides theoretical guidance for the further study of ApPGRP - FD1.

关 键 词：光滑鳖甲 肽聚糖识别蛋白 生物信息学 

分 类 号：Q816[生物学—生物工程]